PDBsum entry 1hf5

Hydrolase

PDB id: 1hf5

Contents

Protein chain

300 a.a.

Ligands

SO4

FCT

GOL ×2

Waters ×510

Superseded by:

1h11

PDB id:

1hf5

Name:

Hydrolase

Title:

2-deoxy-2-fluro-b-d-cellotriosyl/enzyme intermediate complex of the endoglucanase cel5a from bacillus agaradhearans at 1.08 angstrom resolution

Structure:

Endoglucanase b. Chain: a. Fragment: catalytic core domain only. Synonym: cellulase, endo-1,4-beta-glucanase. Engineered: yes

Source:

Bacillus agaradhaerens. Strain: pl2306. Expressed in: bacillus subtilis. Negative.

Resolution:

1.10Å R-factor: 0.112 R-free: 0.129

Authors:

A.Varrot,S.Withers,A.Vasella,M.Schulein,G.J.Davies

Key ref:

A.Varrot et al. Glycoside hydrolases: retaining mechanism at atomic resolution. To be published, .

Date:

28-Nov-00 Release date: 29-Nov-01

Protein chain

O85465  (GUN5_SALAG) -  Endoglucanase 5A from Salipaludibacillus agaradhaerens

Seq: 400 a.a.

Struc: 300 a.a.

Enzyme reactions

• Enzyme class: E.C.3.2.1.4 - cellulase.
• Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.