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PDBsum entry 1gpt
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* Residue conservation analysis
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DOI no:
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Biochemistry
32:715-724
(1993)
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PubMed id:
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Solution structure of gamma 1-H and gamma 1-P thionins from barley and wheat endosperm determined by 1H-NMR: a structural motif common to toxic arthropod proteins.
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M.Bruix,
M.A.Jiménez,
J.Santoro,
C.González,
F.J.Colilla,
E.Méndez,
M.Rico.
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ABSTRACT
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The complete assignment of the proton NMR spectra of the homologous gamma
1-hordothionin and gamma 1-purothionin (47 amino acids, 4 disulfide bridges)
from barley and wheat, respectively, has been performed by two-dimensional
sequence-specific methods. A total of 299 proton-proton distance constraints for
gamma 1-H and 285 for gamma 1-P derived from NOESY spectra have been used to
calculate the three-dimensional solution structures. Initial structures have
been generated by distance geometry methods and further refined by dynamical
simulated annealing calculations. Both proteins show identical secondary and
tertiary structure with a well-defined triple-stranded antiparallel beta-sheet
(residues 1-6, 31-34, and 39-47), an alpha-helix (residues 16-28), and the
corresponding connecting loops. Three disulfide bridges are located in the
hydrophobic core holding together the alpha-helix and the beta-sheet and forming
a cysteine-stabilized alpha-helical (CSH) motif. Moreover, a clustering of
positive charges is observed on the face of the beta-sheet opposite to the
helix. The three-dimensional structures of the gamma-thionins differ remarkably
from plant alpha- and beta-thionins and crambin. However, they show a higher
structural analogy with scorpion toxins and insect defensins which also present
the CSH motif.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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H.U.Stotz,
J.G.Thomson,
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Proteins,
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PDB code:
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M.E.Moghaddam,
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Proteins,
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PDB code:
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Y.S.Shiau,
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Bioinformatics,
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A protective role for the embryo surrounding region of the maize endosperm, as evidenced by the characterisation of ZmESR-6, a defensin gene specifically expressed in this region.
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Plant Mol Biol,
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P.B.Pelegrini,
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Proteins,
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G.Nicastro,
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Solution structure of crotamine, a Na+ channel affecting toxin from Crotalus durissus terrificus venom.
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Eur J Biochem,
270,
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PDB code:
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M.Mishima,
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Structure of the male determinant factor for Brassica self-incompatibility.
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J Biol Chem,
278,
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PDB code:
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P.Da Silva,
L.Jouvensal,
M.Lamberty,
P.Bulet,
A.Caille,
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Solution structure of termicin, an antimicrobial peptide from the termite Pseudacanthotermes spiniger.
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Protein Sci,
12,
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PDB code:
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F.R.Melo,
D.J.Rigden,
O.L.Franco,
L.V.Mello,
M.B.Ary,
M.F.Grossi de Sá,
and
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Inhibition of trypsin by cowpea thionin: characterization, molecular modeling, and docking.
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Proteins,
48,
311-319.
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H.Hemmi,
T.Yoshida,
T.Kumazaki,
N.Nemoto,
J.Hasegawa,
F.Nishioka,
Y.Kyogoku,
H.Yokosawa,
and
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(2002).
Solution structure of ascidian trypsin inhibitor determined by nuclear magnetic resonance spectroscopy.
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Biochemistry,
41,
10657-10664.
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PDB code:
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I.Huys,
K.Dyason,
E.Waelkens,
F.Verdonck,
J.van Zyl,
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G.J.Müller,
J.van der Walt,
E.Clynen,
L.Schoofs,
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(2002).
Purification, characterization and biosynthesis of parabutoxin 3, a component of Parabuthus transvaalicus venom.
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Eur J Biochem,
269,
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O.L.Franco,
D.J.Rigden,
F.R.Melo,
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Plant alpha-amylase inhibitors and their interaction with insect alpha-amylases.
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Roles of disulfide bridges in scorpion toxin BmK M1 analyzed by mutagenesis.
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J Pept Res,
60,
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L.Vila,
V.Lacadena,
P.Fontanet,
A.Martinez del Pozo,
and
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(2001).
A protein from the mold Aspergillus giganteus is a potent inhibitor of fungal plant pathogens.
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Mol Plant Microbe Interact,
14,
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A.J.De Lucca
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C.Landon,
A.Pajon,
F.Vovelle,
and
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(2000).
The active site of drosomycin, a small insect antifungal protein, delineated by comparison with the modeled structure of Rs-AFP2, a plant antifungal protein.
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J Pept Res,
56,
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Solution structure of a conformationally constrained Arg-Gly-Asp-like motif inserted into the alpha/beta scaffold of leiurotoxin I.
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The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance.
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Proteins,
37,
388-403.
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PDB code:
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G.Gao,
J.Dai,
M.Ding,
G.Hellekant,
J.Wang,
and
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Studies on solution NMR structure of brazzein : Secondary structure and molecular scaffold.
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J.M.Schröder
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Epithelial peptide antibiotics.
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Biochem Pharmacol,
57,
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M.Delepierre,
and
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(1999).
Scorpion toxins specific for Na+-channels.
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Eur J Biochem,
264,
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Proteins,
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C.Vita,
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Novel miniproteins engineered by the transfer of active sites to small natural scaffolds.
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Biopolymers,
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E.Drakopoulou,
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V.Aniort,
F.Bouet,
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and
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Consequence of the removal of evolutionary conserved disulfide bridges on the structure and function of charybdotoxin and evidence that particular cysteine spacings govern specific disulfide bond formation.
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Biochemistry,
37,
1292-1301.
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F.García-Olmedo,
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J.M.Alamillo,
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Plant defense peptides.
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Biopolymers,
47,
479-491.
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J.E.Caldwell,
F.Abildgaard,
Z.Dzakula,
D.Ming,
G.Hellekant,
and
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(1998).
Solution structure of the thermostable sweet-tasting protein brazzein.
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Nat Struct Biol,
5,
427-431.
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PDB codes:
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J.L.Dimarcq,
P.Bulet,
C.Hetru,
and
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Cysteine-rich antimicrobial peptides in invertebrates.
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Biopolymers,
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L.Cavallarin,
D.Andreu,
and
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Cecropin A-derived peptides are potent inhibitors of fungal plant pathogens.
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R.E.Hancock,
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Cationic peptides: a new source of antibiotics.
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Trends Biotechnol,
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Y.M.Lee,
B.A.Thomas,
R.Shimizu,
M.Kuroda,
A.D.Jones,
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B.D.Hammock
(1998).
Rapid purification and molecular modeling of AaIT peptides from venom of Androctonus australis.
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Arch Insect Biochem Physiol,
38,
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C.Landon,
P.Sodano,
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C.Kopeyan,
H.Rochat,
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Refined solution structure of the anti-mammal and anti-insect LqqIII scorpion toxin: comparison with other scorpion toxins.
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C.Landon,
P.Sodano,
C.Hetru,
J.Hoffmann,
and
M.Ptak
(1997).
Solution structure of drosomycin, the first inducible antifungal protein from insects.
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Protein Sci,
6,
1878-1884.
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PDB code:
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E.Adjadj,
V.Naudat,
E.Quiniou,
D.Wouters,
P.Sautière,
and
C.T.Craescu
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Solution structure of Lqh-8/6, a toxin-like peptide from a scorpion venom--structural heterogeneity induced by proline cis/trans isomerization.
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Eur J Biochem,
246,
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G.W.De Samblanx,
I.J.Goderis,
K.Thevissen,
R.Raemaekers,
F.Fant,
F.Borremans,
D.P.Acland,
R.W.Osborn,
S.Patel,
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Mutational analysis of a plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity.
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J Biol Chem,
272,
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J.Song,
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C.Vita,
and
A.Ménez
(1997).
NMR solution structure of a two-disulfide derivative of charybdotoxin: structural evidence for conservation of scorpion toxin alpha/beta motif and its hydrophobic side chain packing.
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Biochemistry,
36,
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PDB code:
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Y.Zhang,
and
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Eur J Biochem,
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PDB code:
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J Biol Chem,
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Eur J Biochem,
239,
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Biochemistry,
35,
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PDB code:
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K.Thevissen,
A.Ghazi,
G.W.De Samblanx,
C.Brownlee,
R.W.Osborn,
and
W.F.Broekaert
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Fungal membrane responses induced by plant defensins and thionins.
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J Biol Chem,
271,
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S.Rudolph-Böhner,
and
F.Siedler
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Oxidative folding of cystine-rich peptides vs regioselective cysteine pairing strategies.
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Biopolymers,
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E.Drakopoulou,
B.Gilquin,
C.Vita,
and
A.Ménez
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Transfer of a beta-hairpin from the functional site of snake curaremimetic toxins to the alpha/beta scaffold of scorpion toxins: three-dimensional solution structure of the chimeric protein.
|
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Biochemistry,
35,
8535-8543.
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PDB code:
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C.Vita,
C.Roumestand,
F.Toma,
and
A.Ménez
(1995).
Scorpion toxins as natural scaffolds for protein engineering.
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Proc Natl Acad Sci U S A,
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L.Morhy,
G.Marino,
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C.González,
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F.Soriano,
A.Rocher,
E.Méndez,
and
M.Rico
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1H-nmr studies on the structure of a new thionin from barley endosperm.
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| |
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A common structural motif incorporating a cystine knot and a triple-stranded beta-sheet in toxic and inhibitory polypeptides.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
