spacer
spacer

PDBsum entry 1ea3
Go to PDB code: 
protein Protein-protein interface(s) links
Influenza virus PDB id
1ea3

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chains
157 a.a.
Waters ×81
PDB id:
1ea3
Name: Influenza virus
Title: Influenza virus m1 protein
Structure: Matrix protein m1. Chain: a, b. Fragment: n-terminal domain residues 1-164. Engineered: yes. Other_details: n-terminal domain residue 1-164
Source: Influenza a virus. Organism_taxid: 11320. Strain: a/pr/8/34. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.30Å     R-factor:   0.242     R-free:   0.313
Authors: S.Arzt
Key ref: S.Arzt et al. (2001). Combined results from solution studies on intact influenza virus M1 protein and from a new crystal form of its N-terminal domain show that M1 is an elongated monomer. Virology, 279, 439-446. PubMed id: 11162800
Date:
03-Nov-00     Release date:   26-Apr-01    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P03485  (M1_I34A1) -  Matrix protein 1 from Influenza A virus (strain A/Puerto Rico/8/1934 H1N1)
Seq:
Struc: 		252 a.a.
157 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
Virology 279:439-446 (2001)
PubMed id: 11162800  
 
 
Combined results from solution studies on intact influenza virus M1 protein and from a new crystal form of its N-terminal domain show that M1 is an elongated monomer.
S.Arzt, F.Baudin, A.Barge, P.Timmins, W.P.Burmeister, R.W.Ruigrok.
 
  ABSTRACT  
 
The amino-terminal domain of influenza A virus matrix protein (residues 1-164) was crystallized at pH 7 into a new crystal form in space group P1. This packing of the protein implies that M1(1-164) was monomeric in solution when it crystallized. Otherwise, the structure of the M1 fragment in the pH 7 crystals was the same as the monomers in crystals formed at pH 4 where crystal packing resulted in dimer formation [B. Sha and M. Luo, 1997, Nature Struct. Biol. 4, 239-244]. Analysis of intact M1 protein, the N-terminal domain, and the remaining C-terminal fragment (residues 165-252) in solution also showed that the N-terminal domain was monomeric with the same dimensions as determined from the crystal structure. Intact M1 protein was also monomeric but with an elongated shape due to the presence of the C-terminal part. Circular dichroism showed that the C-terminal part of M1 contained helical structure. A model for soluble M1 is presented, based on the assumption that the C-terminal domain is spherical, in which the N- and C-terminal domains are connected by a linker sequence which is available for proteolytic attack.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20498070 L.J.Calder, S.Wasilewski, J.A.Berriman, and P.B.Rosenthal (2010).
Structural organization of a filamentous influenza A virus.
  Proc Natl Acad Sci U S A, 107, 10685-10690.  
19656971 J.Zhang, G.Li, X.Liu, Z.Wang, W.Liu, and X.Ye (2009).
Influenza A virus M1 blocks the classical complement pathway through interacting with C1qA.
  J Gen Virol, 90, 2751-2758.  
19521658 S.Huang, J.Chen, H.Wang, B.Sun, H.Wang, Z.Zhang, X.Zhang, and Z.Chen (2009).
Influenza A virus matrix protein 1 interacts with hTFIIIC102-s, a short isoform of the polypeptide 3 subunit of human general transcription factor IIIC.
  Arch Virol, 154, 1101-1110.  
18672693 I.u.A.Smirnova, L.V.Kordiukova, M.V.Serebriakova, I.I.u.Filippova, E.N.Lysogorskaia, and L.A.Baratova (2008).
[Flu virion as a substrate for proteolytic enzymes]
  Bioorg Khim, 34, 409-415.  
18725937 T.T.Lam, C.C.Hon, O.G.Pybus, S.L.Kosakovsky Pond, R.T.Wong, C.W.Yip, F.Zeng, and F.C.Leung (2008).
Evolutionary and transmission dynamics of reassortant H5N1 influenza virus in Indonesia.
  PLoS Pathog, 4, e1000130.  
17622633 S.L.Noton, E.Medcalf, D.Fisher, A.E.Mullin, D.Elton, and P.Digard (2007).
Identification of the domains of the influenza A virus M1 matrix protein required for NP binding, oligomerization and incorporation into virions.
  J Gen Virol, 88, 2280-2290.  
16474136 E.K.Hui, S.Barman, D.H.Tang, B.France, and D.P.Nayak (2006).
YRKL sequence of influenza virus M1 functions as the L domain motif and interacts with VPS28 and Cdc42.
  J Virol, 80, 2291-2308.  
16227287 B.J.Chen, M.Takeda, and R.A.Lamb (2005).
Influenza virus hemagglutinin (H3 subtype) requires palmitoylation of its cytoplasmic tail for assembly: M1 proteins of two subtypes differ in their ability to support assembly.
  J Virol, 79, 13673-13684.  
15780878 D.Pasdeloup, N.Poisson, H.Raux, Y.Gaudin, R.W.Ruigrok, and D.Blondel (2005).
Nucleocytoplasmic shuttling of the rabies virus P protein requires a nuclear localization signal and a CRM1-dependent nuclear export signal.
  Virology, 334, 284-293.  
16103152 E.Hoffmann, K.Mahmood, Z.Chen, C.F.Yang, J.Spaete, H.B.Greenberg, M.L.Herlocher, H.Jin, and G.Kemble (2005).
Multiple gene segments control the temperature sensitivity and attenuation phenotypes of ca B/Ann Arbor/1/66.
  J Virol, 79, 11014-11021.  
15661164 I.Garcia-Robles, H.Akarsu, C.W.Müller, R.W.Ruigrok, and F.Baudin (2005).
Interaction of influenza virus proteins with nucleosomes.
  Virology, 332, 329-336.  
15613353 L.M.Burleigh, L.J.Calder, J.J.Skehel, and D.A.Steinhauer (2005).
Influenza a viruses with mutations in the m1 helix six domain display a wide variety of morphological phenotypes.
  J Virol, 79, 1262-1270.  
15731254 M.F.McCown, and A.Pekosz (2005).
The influenza A virus M2 cytoplasmic tail is required for infectious virus production and efficient genome packaging.
  J Virol, 79, 3595-3605.  
15108720 J.Timmins, R.W.Ruigrok, and W.Weissenhorn (2004).
Structural studies on the Ebola virus matrix protein VP40 indicate that matrix proteins of enveloped RNA viruses are analogues but not homologues.
  FEMS Microbiol Lett, 233, 179-186.  
12768027 E.K.Hui, S.Barman, T.Y.Yang, and D.P.Nayak (2003).
Basic residues of the helix six domain of influenza virus M1 involved in nuclear translocation of M1 can be replaced by PTAP and YPDL late assembly domain motifs.
  J Virol, 77, 7078-7092.  
12970177 H.Akarsu, W.P.Burmeister, C.Petosa, I.Petit, C.W.Müller, R.W.Ruigrok, and F.Baudin (2003).
Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2).
  EMBO J, 22, 4646-4655.
PDB code: 1pd3
12065402 M.Gaudier, Y.Gaudin, and M.Knossow (2002).
Crystal structure of vesicular stomatitis virus matrix protein.
  EMBO J, 21, 2886-2892.
PDB code: 1lg7
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

spacer
spacer