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PDBsum entry 1e9m
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Iron-sulfur protein PDB id
1e9m

 

 

 

 

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Contents
Protein chain
106 a.a. *
Ligands
FES
Waters ×57
* Residue conservation analysis
PDB id:
1e9m
Name: Iron-sulfur protein
Title: Ferredoxin vi from rhodobacter capsulatus
Structure: Ferredoxin vi. Chain: a. Engineered: yes
Source: Rhodobacter capsulatus. Organism_taxid: 1061. Strain: b10. Gene: fdxe. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.07Å     R-factor:   0.196     R-free:   0.214
Authors: G.Sainz,J.Armengaud,V.Stojanoff,N.Sanishvili,Y.Jouanneau,S.Larry
Key ref:
J.Armengaud et al. (2001). Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus. Acta Crystallogr D Biol Crystallogr, 57, 301-303. PubMed id: 11173487 DOI: 10.1107/S0907444900017832
Date:
24-Oct-00     Release date:   09-Apr-01    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P80306  (FER6_RHOCA) -  Ferredoxin-6 from Rhodobacter capsulatus
Seq:
Struc: 		107 a.a.
106 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
DOI no: 10.1107/S0907444900017832 Acta Crystallogr D Biol Crystallogr 57:301-303 (2001)
PubMed id: 11173487  
 
 
Crystallization and preliminary X-ray diffraction analysis of a [2Fe-2S] ferredoxin (FdVI) from Rhodobacter capsulatus.
J.Armengaud, G.Sainz, Y.Jouanneau, L.C.Sieker.
 
  ABSTRACT  
 
ferredoxin found in the photosynthetic bacterium Rhodobacter capsulatus has been purified in recombinant form from Escherichia coli. This protein, called FdVI, resembles ferredoxins involved in iron-sulfur cluster biosynthesis in various prokaryotic and eukaryotic cells. Purified recombinant FdVI was recovered in high yields and appeared to be indistinguishable from the genuine R. capsulatus ferredoxin based on UV-visible absorption and EPR spectroscopy and mass spectrometry. FdVI has been crystallized in the oxidized state by a sitting-drop vapour-diffusion technique using sodium formate as precipitant. Seeding larger drops from a previous hanging-drop-grown small crystal resulted in the formation of long red-brown prismatic needles. Preliminary X-ray diffraction analysis indicated that FdVI crystals are orthorhombic and belong to the space group P2(1)2(1)2(1), with unit-cell parameters a = 45.87, b = 49.83, c = 54.29 A.
 
  Selected figure(s)  
 
Figure 2.
Figure 2 Recombinant FdVI crystals.
 
  The above figure is reprinted by permission from the IUCr: Acta Crystallogr D Biol Crystallogr (2001, 57, 301-303) copyright 2001.  
  Figure was selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
16402206 G.Sainz, J.Jakoncic, L.C.Sieker, V.Stojanoff, N.Sanishvili, M.Asso, P.Bertrand, J.Armengaud, and Y.Jouanneau (2006).
Structure of a [2Fe-2S] ferredoxin from Rhodobacter capsulatus likely involved in Fe-S cluster biogenesis and conformational changes observed upon reduction.
  J Biol Inorg Chem, 11, 235-246.
PDB code: 1uwm
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

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