spacer
spacer

PDBsum entry 1cto
Go to PDB code: 
protein links
Binding protein PDB id
1cto

 

 

 

 

Loading ...

 
JSmol PyMol  
Contents
Protein chain
109 a.a. *
* Residue conservation analysis
PDB id:
1cto
Name: Binding protein
Title: Nmr structure of thE C-terminal domain of the ligand-binding region of murine granulocyte colony-stimulating factor receptor, minimized average structure
Structure: Granulocyte colony-stimulating factor receptor. Chain: a. Fragment: c-terminal domain of the ligand-binding region. Engineered: yes. Mutation: yes
Source: Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: expressed as a fusion protein with maltose binding protein
NMR struc: 1 models
Authors: K.Yamasaki,S.Naito,H.Anaguchi,T.Ohkubo,Y.Ota
Key ref: K.Yamasaki et al. (1997). Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand. Nat Struct Biol, 4, 498-504. PubMed id: 9187659
Date:
25-Sep-96     Release date:   22-Oct-97    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P40223  (CSF3R_MOUSE) -  Granulocyte colony-stimulating factor receptor from Mus musculus
Seq:
Struc: 		 
Seq:
Struc: 		837 a.a.
109 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 

 
Nat Struct Biol 4:498-504 (1997)
PubMed id: 9187659  
 
 
Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand.
K.Yamasaki, S.Naito, H.Anaguchi, T.Ohkubo, Y.Ota.
 
  ABSTRACT  
 
We have determined the NMR structure of a ligand-binding domain of the granulocyte colony-stimulating factor (G-CSF) receptor, containing the highly conserved WSxWS motif. The domain consists of seven beta-strands with the fibronectin type III-like topology seen in several cytokine receptors. Comparisons between the spectra of the 15N-labelled domain with and without G-CSF indicate that the major ligand-recognition site is on the FG loop just upstream of the WSxWS sequence, and not on the BC loop which is mainly used in the growth hormone system. The WSxWS residues are suggested to contribute to ligand-recognition and to the protein architecture of the G-CSF receptor.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20874424 M.Saukko, Y.A.Kesäniemi, and O.Ukkola (2010).
Leptin receptor Lys109Arg and Gln223Arg polymorphisms are associated with early atherosclerosis.
  Metab Syndr Relat Disord, 8, 425-430.  
16540470 F.Peelman, H.Iserentant, A.S.De Smet, J.Vandekerckhove, L.Zabeau, and J.Tavernier (2006).
Mapping of binding site III in the leptin receptor and modeling of a hexameric leptin.leptin receptor complex.
  J Biol Chem, 281, 15496-15504.  
14557255 A.Schwantner, A.J.Dingley, S.Ozbek, S.Rose-John, and J.Grötzinger (2004).
Direct determination of the interleukin-6 binding epitope of the interleukin-6 receptor by NMR spectroscopy.
  J Biol Chem, 279, 571-576.  
12440146 K.Morikawa (2002).
[Ligand recognition mechanism of G-CSF receptor and metabotropic glutamate receptor]
  Yakugaku Zasshi, 122, 855-868.  
10681548 C.Olivier, P.Auguste, M.Chabbert, E.Lelièvre, S.Chevalier, and H.Gascan (2000).
Identification of a gp130 cytokine receptor critical site involved in oncostatin M response.
  J Biol Chem, 275, 5648-5656.  
10449521 A.C.Ward, Y.M.van Aesch, J.Gits, A.M.Schelen, J.P.de Koning, D.van Leeuwen, M.H.Freedman, and I.P.Touw (1999).
Novel point mutation in the extracellular domain of the granulocyte colony-stimulating factor (G-CSF) receptor in a case of severe congenital neutropenia hyporesponsive to G-CSF treatment.
  J Exp Med, 190, 497-507.  
10567387 A.Haman, C.Cadieux, B.Wilkes, T.Hercus, A.Lopez, S.Clark, and T.Hoang (1999).
Molecular determinants of the granulocyte-macrophage colony-stimulating factor receptor complex assembly.
  J Biol Chem, 274, 34155-34163.  
10364174 J.E.Layton, G.Shimamoto, T.Osslund, A.Hammacher, D.K.Smith, H.R.Treutlein, and T.Boone (1999).
Interaction of granulocyte colony-stimulating factor (G-CSF) with its receptor. Evidence that Glu19 of G-CSF interacts with Arg288 of the receptor.
  J Biol Chem, 274, 17445-17451.  
10494829 J.Grötzinger, T.Kernebeck, K.J.Kallen, and S.Rose-John (1999).
IL-6 type cytokine receptor complexes: hexamer, tetramer or both?
  Biol Chem, 380, 803-813.  
10318834 S.A.Middleton, F.P.Barbone, D.L.Johnson, R.L.Thurmond, Y.You, F.J.McMahon, R.Jin, O.Livnah, J.Tullai, F.X.Farrell, M.A.Goldsmith, I.A.Wilson, and L.K.Jolliffe (1999).
Shared and unique determinants of the erythropoietin (EPO) receptor are important for binding EPO and EPO mimetic peptide.
  J Biol Chem, 274, 14163-14169.  
  10210178 T.Kernebeck, S.Pflanz, G.Müller-Newen, G.Kurapkat, R.M.Scheek, K.Dijkstra, P.C.Heinrich, A.Wollmer, S.Grzesiek, and J.Grötzinger (1999).
The signal transducer gp130: solution structure of the carboxy-terminal domain of the cytokine receptor homology region.
  Protein Sci, 8, 5.
PDB code: 1bj8
9501088 J.Bravo, D.Staunton, J.K.Heath, and E.Y.Jones (1998).
Crystal structure of a cytokine-binding region of gp130.
  EMBO J, 17, 1665-1674.
PDB code: 1bqu
9368043 J.E.Layton, J.Iaria, D.K.Smith, and H.R.Treutlein (1997).
Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis.
  J Biol Chem, 272, 29735-29741.
PDB code: 1az7
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.

 

spacer
spacer