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PDBsum entry 1c0c
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Contents |
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* Residue conservation analysis
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Enzyme class:
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E.C.4.6.1.18
- pancreatic ribonuclease.
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Reaction:
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1.
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an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
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2.
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an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA]
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DOI no:
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Protein Sci
8:2033-2040
(1999)
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PubMed id:
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X-ray crystal structures of a severely desiccated protein.
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J.A.Bell.
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ABSTRACT
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Unlike most protein crystals, form IX of bovine pancreatic ribonuclease A
diffracts well when severely dehydrated. Crystal structures have been solved
after 2.5 and 4 days of desiccation with CaSO4, at 1.9 and 2.0 A resolution,
respectively. The two desiccated structures are very similar. An RMS
displacement of 1.6 A is observed for main-chain atoms in each structure when
compared to the hydrated crystal structure with some large rearrangements
observed in loop regions. The structural changes are the result of
intermolecular contacts formed by strong electrostatic interactions in the
absence of a high dielectric medium. The electron density is very diffuse for
some surface loops, consistent with a very disordered structure. This disorder
is related to the conformational changes. These results help explain
conformational changes during the lyophilization of protein and the associated
phenomena of denaturation and molecular memory.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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J.Rabone,
Y.F.Yue,
S.Y.Chong,
K.C.Stylianou,
J.Bacsa,
D.Bradshaw,
G.R.Darling,
N.G.Berry,
Y.Z.Khimyak,
A.Y.Ganin,
P.Wiper,
J.B.Claridge,
and
M.J.Rosseinsky
(2010).
An adaptable peptide-based porous material.
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Science,
329,
1053-1057.
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V.Gupta,
R.K.Gupta,
G.Khare,
D.M.Salunke,
A.Surolia,
and
A.K.Tyagi
(2010).
Structural ordering of disordered ligand-binding loops of biotin protein ligase into active conformations as a consequence of dehydration.
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PLoS One,
5,
e9222.
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PDB codes:
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B.L.Simons,
H.Kaplan,
S.M.Fournier,
T.Cyr,
and
M.A.Hefford
(2007).
A novel cross-linked RNase A dimer with enhanced enzymatic properties.
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Proteins,
66,
183-195.
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K.Harata,
and
T.Akiba
(2006).
Structural phase transition of monoclinic crystals of hen egg-white lysozyme.
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Acta Crystallogr D Biol Crystallogr,
62,
375-382.
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PDB codes:
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J.J.Hill,
E.Y.Shalaev,
and
G.Zografi
(2005).
Thermodynamic and dynamic factors involved in the stability of native protein structure in amorphous solids in relation to levels of hydration.
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J Pharm Sci,
94,
1636-1667.
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K.Harata,
and
T.Akiba
(2004).
Phase transition of triclinic hen egg-white lysozyme crystal associated with sodium binding.
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Acta Crystallogr D Biol Crystallogr,
60,
630-637.
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PDB codes:
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G.D.Smith,
and
R.H.Blessing
(2003).
Lessons from an aged, dried crystal of T(6) human insulin.
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Acta Crystallogr D Biol Crystallogr,
59,
1384-1394.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
