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PDBsum entry 1b3e
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Iron transport
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PDB id
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1b3e
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Iron transport
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Title:
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Human serum transferrin, n-terminal lobe, expressed in pichia pastoris
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Structure:
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Protein (serum transferrin). Chain: a. Fragment: n-terminal lobe. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: pichia pastoris. Expression_system_taxid: 4922
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Resolution:
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2.50Å
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R-factor:
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0.177
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R-free:
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0.255
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Authors:
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M.C.Bewley,B.M.Tam,J.Grewal,S.He,S.Shewry,M.E.P.Murphy,A.B.Mason, R.C.Woodworth,E.N.Baker,R.T.A.Macgillivray
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Key ref:
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M.C.Bewley
et al.
(1999).
X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32.
Biochemistry,
38,
2535-2541.
PubMed id:
DOI:
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Date:
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09-Dec-98
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Release date:
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26-Mar-99
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PROCHECK
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Headers
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References
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P02787
(TRFE_HUMAN) -
Serotransferrin from Homo sapiens
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Seq:
Struc:
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698 a.a.
330 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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DOI no:
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Biochemistry
38:2535-2541
(1999)
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PubMed id:
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X-ray crystallography and mass spectroscopy reveal that the N-lobe of human transferrin expressed in Pichia pastoris is folded correctly but is glycosylated on serine-32.
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M.C.Bewley,
B.M.Tam,
J.Grewal,
S.He,
S.Shewry,
M.E.Murphy,
A.B.Mason,
R.C.Woodworth,
E.N.Baker,
R.T.MacGillivray.
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ABSTRACT
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The ferric form of the N-lobe of human serum transferrin (Fe(III)-hTF/2N) has
been expressed at high levels in Pichia pastoris. The Fe(III)-hTF/2N was
crystallized in the space group P41212, and X-ray crystallography was used to
solve the structure of the recombinant protein at 2.5 A resolution. This
represents only the second P. pastoris-derived protein structure determined to
date, and allows the comparison of the structures of recombinant Fe(III)-hTF/2N
expressed in P. pastoris and mammalian cells with serum-derived transferrin. The
polypeptide folding pattern is essentially identical in all of the three
proteins. Mass spectroscopic analyses of P. pastoris- hTF/2N and proteolytically
derived fragments revealed glycosylation of Ser-32 with a single hexose. This
represents the first localization of an O-linked glycan in a P. pastoris-derived
protein. Because of its distance from the iron-binding site, glycosylation of
Ser-32 should not affect the iron-binding properties of hTF/2N expressed in P.
pastoris, making this an excellent expression system for the production of
hTF/2N.
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Literature references that cite this PDB file's key reference
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Google scholar
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PubMed id
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Reference
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R.K.Huang,
N.F.Steinmetz,
C.Y.Fu,
M.Manchester,
and
J.E.Johnson
(2011).
Transferrin-mediated targeting of bacteriophage HK97 nanoparticles into tumor cells.
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Nanomedicine (Lond),
6,
55-68.
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A.Jeanson,
M.Ferrand,
H.Funke,
C.Hennig,
P.Moisy,
P.L.Solari,
C.Vidaud,
and
C.Den Auwer
(2010).
The role of transferrin in actinide(IV) uptake: comparison with iron(III).
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Chemistry,
16,
1378-1387.
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C.J.Finnis,
T.Payne,
J.Hay,
N.Dodsworth,
D.Wilkinson,
P.Morton,
M.J.Saxton,
D.J.Tooth,
R.W.Evans,
H.Goldenberg,
B.Scheiber-Mojdehkar,
N.Ternes,
and
D.Sleep
(2010).
High-level production of animal-free recombinant transferrin from saccharomyces cerevisiae.
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Microb Cell Fact,
9,
87.
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P.Guha Thakurta,
D.Choudhury,
R.Dasgupta,
and
J.K.Dattagupta
(2003).
Structure of diferric hen serum transferrin at 2.8 A resolution.
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Acta Crystallogr D Biol Crystallogr,
59,
1773-1781.
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PDB code:
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S.Oztürk,
B.Cirakoglu,
and
E.Bermek
(2003).
Partial characterization of the human serum transferrin epitope reactive with the monoclonal antibody TRC-2.
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Hybrid Hybridomics,
22,
165-171.
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D.R.Hall,
J.M.Hadden,
G.A.Leonard,
S.Bailey,
M.Neu,
M.Winn,
and
P.F.Lindley
(2002).
The crystal and molecular structures of diferric porcine and rabbit serum transferrins at resolutions of 2.15 and 2.60 A, respectively.
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Acta Crystallogr D Biol Crystallogr,
58,
70-80.
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PDB codes:
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A.H.Yang,
R.T.MacGillivray,
J.Chen,
Y.Luo,
Y.Wang,
G.D.Brayer,
A.B.Mason,
R.C.Woodworth,
and
M.E.Murphy
(2000).
Crystal structures of two mutants (K206Q, H207E) of the N-lobe of human transferrin with increased affinity for iron.
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Protein Sci,
9,
49-52.
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PDB codes:
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J.L.Cereghino,
and
J.M.Cregg
(2000).
Heterologous protein expression in the methylotrophic yeast Pichia pastoris.
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FEMS Microbiol Rev,
24,
45-66.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
