PDBsum entry 1b1v

Cytokine

PDB id

1b1v

Loading ...

Contents

			Protein chain

					23 a.a.

PDB id:

1b1v

Links

Name:

Cytokine

Title:

Nmr structure of psp1, plasmatocyte-spreading peptide from pseudoplusia includens

Structure:

Protein (plasmatocyte-spreading peptide). Chain: a. Synonym: psp1. Engineered: yes

Source:

Synthetic: yes. Other_details: the peptide was chemically synthesized. The sequence of this peptide is naturally found in the hemolymph tissue of pseudoplusia includens (soybean looper (moth)).

NMR struc:

30 models

Authors:

B.F.Volkman,K.D.Clark,M.E.Anderson,L.L.Pech,J.L.Markley,M.R.Strand

Key ref:

B.F.Volkman et al. (1999). Structure of the insect cytokine peptide plasmatocyte-spreading peptide 1 from Pseudoplusia includens. J Biol Chem, 274, 4493-4496. PubMed id: 9988679 DOI: 10.1074/jbc.274.8.4493

Date:

23-Nov-98

Release date:

02-Dec-98

PROCHECK

	Headers

	References

Protein chain

O61704 (PSP1_CHRIL) - Plasmatocyte-spreading peptide from Chrysodeixis includens

Seq: Struc:					141 a.a. 23 a.a.

Key:

PfamA domain

Secondary structure

DOI no: 10.1074/jbc.274.8.4493	J Biol Chem 274:4493-4496 (1999)
PubMed id: 9988679

Structure of the insect cytokine peptide plasmatocyte-spreading peptide 1 from Pseudoplusia includens.
B.F.Volkman, M.E.Anderson, K.D.Clark, Y.Hayakawa, M.R.Strand, J.L.Markley.

ABSTRACT

The structure of the recently identified plasmatocyte spreading peptide from the moth Pseudoplusia includens (PSP1) has been determined by NMR spectroscopy. This novel insect cytokine consists of 23 amino acid residues and a single disulfide bond. Torsion angle dynamics calculations utilizing a total of 337 distance constraints yielded an ensemble of 30 structures with an average backbone root mean square deviation for residues 7-22 of 0.18 A from the mean structure. The structure consists of a disordered N-terminal region and a well defined core that is stabilized by numerous hydrophobic interactions and a short beta-hairpin. Structural comparisons confirm that PSP1 adopts an epidermal growth factor (EGF)-like fold with close similarity to the C-terminal subdomain of EGF-like module 5 of human thrombomodulin. The combination of the three-dimensional structure of PSP1 and the extensive literature on EGF-receptor interactions should accelerate the process of identifying the specific residues responsible for receptor binding activity of this family of immunoregulatory peptides.

Selected figure(s)



	Figure 1. Fig. 1. Comparison of the sequences of PSP1 from P. includens, GBP from P. separata, paralytic peptide from M. sexta (PP1), and the C-terminal subdomains of human epidermal growth factor (hEGF) and the hTM5. Highlighted in boldface type are the cysteine, glycine, and tyrosine residues conserved in the EGF-like domain family and the corresponding residues of the insect peptides.		Figure 6. Fig. 6. Ribbon diagram of the minimized average structure of PSP1 with all nonhydrogen side chain atoms shown. The charged side chains of the -hairpin are labeled.

Figures were selected by an automated process.

Literature references that cite this PDB file's key reference

PubMed id

Reference

18004777

K.Anamika, A.Bhattacharya, and N.Srinivasan (2008).
Analysis of the protein kinome of Entamoeba histolytica.

Proteins, 71, 995.

15199957

M.R.Kanost, H.Jiang, and X.Q.Yu (2004).
Innate immune responses of a lepidopteran insect, Manduca sexta.

Immunol Rev, 198, 97.

12655604

Y.Nakahara, Y.Kanamori, M.Kiuchi, and M.Kamimura (2003).
Effects of silkworm paralytic peptide on in vitro hematopoiesis and plasmatocyte spreading.

Arch Insect Biochem Physiol, 52, 163-174.

12434426

J.Song, P.Xu, A.Koutychenko, and F.Ni (2002).
Stability of protein-bound conformations of bioactive peptides: the folded conformation of an epidermal growth factor-like thrombomodulin fragment is similar to that recognized by thrombin.

Biopolymers, 65, 373-386.

11268040

O.Schmidt, U.Theopold, and M.Strand (2001).
Innate immunity and its evasion and suppression by hymenopteran endoparasitoids.

Bioessays, 23, 344-351.

10536049

M.R.Strand, and K.D.Clark (1999).
Plasmatocyte spreading peptide induces spreading of plasmatocytes but represses spreading of granulocytes.

Arch Insect Biochem Physiol, 42, 213-223.

10517164

X.Q.Yu, O.Prakash, and M.R.Kanost (1999).
Structure of a paralytic peptide from an insect, Manduca sexta.

J Pept Res, 54, 256-261.

PDB code:

1hrl

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.