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PDBsum entry 1ae5
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Serine protease homolog
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PDB id
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1ae5
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Serine protease homolog
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Title:
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Human heparin binding protein
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Structure:
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Heparin binding protein. Chain: a. Synonym: cap37, azurocidin. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Cell_line: sf-900. Expressed in: unidentified baculovirus. Expression_system_taxid: 10469. Expression_system_cell_line: sf-900.
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Resolution:
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2.30Å
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R-factor:
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0.192
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R-free:
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0.266
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Authors:
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L.F.Iversen,J.S.Kastrup,S.E.Bjorn,P.B.Rasmussen,F.C.Wiberg, H.J.Flodgaard,I.K.Larsen
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Key ref:
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L.F.Iversen
et al.
(1997).
Structure of HBP, a multifunctional protein with a serine proteinase fold.
Nat Struct Biol,
4,
265-268.
PubMed id:
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Date:
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05-Mar-97
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Release date:
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11-Mar-98
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PROCHECK
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Headers
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References
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P20160
(CAP7_HUMAN) -
Azurocidin from Homo sapiens
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Seq:
Struc:
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251 a.a.
223 a.a.
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Key: |
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Secondary structure |
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CATH domain |
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Nat Struct Biol
4:265-268
(1997)
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PubMed id:
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Structure of HBP, a multifunctional protein with a serine proteinase fold.
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L.F.Iversen,
J.S.Kastrup,
S.E.Bjørn,
P.B.Rasmussen,
F.C.Wiberg,
H.J.Flodgaard,
I.K.Larsen.
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ABSTRACT
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The structure of human heparin binding protein reveals that the serine
proteinase fold has been used as a scaffold for a multifunctional protein with
antibacterial activity, monocyte and t-cell activating properties and endotoxin
and heparin binding capacity.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.Olczak,
K.Indyk,
and
T.Olczak
(2008).
Reconstitution of human azurocidin catalytic triad and proteolytic activity by site-directed mutagenesis.
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Biol Chem,
389,
955-962.
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S.Voss,
S.Welte,
M.Fotin-Mleczek,
R.Fischer,
A.J.Ulmer,
G.Jung,
K.H.Wiesmüller,
and
R.Brock
(2006).
A CD14 domain with lipopolysaccharide-binding and -neutralizing activity.
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Chembiochem,
7,
275-286.
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A.E.Todd,
C.A.Orengo,
and
J.M.Thornton
(2002).
Sequence and structural differences between enzyme and nonenzyme homologs.
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Structure,
10,
1435-1451.
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D.McCabe,
T.Cukierman,
and
J.E.Gabay
(2002).
Basic residues in azurocidin/HBP contribute to both heparin binding and antimicrobial activity.
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J Biol Chem,
277,
27477-27488.
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H.Bosshart,
and
M.Heinzelmann
(2002).
Arginine-rich cationic polypeptides amplify lipopolysaccharide-induced monocyte activation.
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Infect Immun,
70,
6904-6910.
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J.S.Kastrup,
V.Linde,
A.K.Pedersen,
B.Stoffer,
L.F.Iversen,
I.K.Larsen,
P.B.Rasmussen,
H.J.Flodgaard,
and
S.E.Bjørn
(2001).
Two mutants of human heparin binding protein (CAP37): toward the understanding of the nature of lipid A/LPS and BPTI binding.
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Proteins,
42,
442-451.
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PDB codes:
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D.Devos,
and
A.Valencia
(2000).
Practical limits of function prediction.
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Proteins,
41,
98.
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F.Shakib,
and
R.Furmonaviciene
(2000).
The significance of enzymic and other biological activities of proteins in relation to their capacity to serve as allergens.
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Clin Exp Allergy,
30,
1056-1057.
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M.Heinzelmann,
M.A.Mercer-Jones,
J.Peyton,
H.Flodgaard,
and
W.G.Cheadle
(2000).
Heparin binding protein increases survival in murine fecal peritonitis.
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Crit Care Med,
28,
2926-2931.
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V.Frecer,
B.Ho,
and
J.L.Ding
(2000).
Interpretation of biological activity data of bacterial endotoxins by simple molecular models of mechanism of action.
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Eur J Biochem,
267,
837-852.
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A.M.Olofsson,
M.Vestberg,
H.Herwald,
J.Rygaard,
G.David,
K.E.Arfors,
V.Linde,
H.Flodgaard,
J.Dedio,
W.Müller-Esterl,
and
E.Lundgren-Akerlund
(1999).
Heparin-binding protein targeted to mitochondrial compartments protects endothelial cells from apoptosis.
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J Clin Invest,
104,
885-894.
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H.Czapinska,
and
J.Otlewski
(1999).
Structural and energetic determinants of the S1-site specificity in serine proteases.
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Eur J Biochem,
260,
571-595.
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L.F.Iversen,
J.S.Kastrup,
S.E.Bjørn,
F.C.Wiberg,
I.K.Larsen,
H.J.Flodgaard,
and
P.B.Rasmussen
(1999).
Structure and function of the N-linked glycans of HBP/CAP37/azurocidin: crystal structure determination and biological characterization of nonglycosylated HBP.
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Protein Sci,
8,
2019-2026.
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M.Heinzelmann,
H.C.Polk,
and
F.N.Miller
(1998).
Modulation of lipopolysaccharide-induced monocyte activation by heparin-binding protein and fucoidan.
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Infect Immun,
66,
5842-5847.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
