PDBsum entry 1a2f

Oxidoreductase

PDB id: 1a2f

Contents

Protein chain

291 a.a. *

Ligands

HEM

Waters ×107

* Residue conservation analysis

PDB id:

1a2f

Name:

Oxidoreductase

Title:

Probing the strength and character of an asp-his-x hydrogen bond by introducing buried charges

Structure:

CytochromE C peroxidase. Chain: a. Synonym: ccp/m172k. Engineered: yes. Mutation: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Cell_line: bl21. Organelle: mitochondria. Gene: ccp. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.10Å R-factor: 0.184

Authors:

Y.Cao,D.B.Goodin,D.E.Mcree

Key ref:

Y.Cao et al. Probing the strength and character of an asp-His-X hydrogen bond by introducing buried charges. To be published, .

Date:

02-Jan-98 Release date: 25-Nov-98

Protein chain

P00431  (CCPR_YEAST) -  Cytochrome c peroxidase, mitochondrial from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 361 a.a.

Struc: 291 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.11.1.5 - cytochrome-c peroxidase.
• Reaction: 2 Fe(II)-[cytochrome c] + H2O2 + 2 H⁺ = 2 Fe(III)-[cytochrome c] + 2 H2O
• Cofactor: Heme

Heme (Bound ligand (Het Group name = HEM) matches with 95.45% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site