PDBsum entry 1to9

Biosynthetic protein

PDB id

1to9

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Contents

			Protein chains

					225 a.a. *

			Ligands

					HMH ×2

			Waters ×29

* Residue conservation analysis

PDB id:

1to9

Links

Name:

Biosynthetic protein

Title:

Crystal structure of thi-4 protein from bacillus subtilis

Structure:

Thi-4 protein. Chain: a. Engineered: yes. Thi-4 protein. Chain: b. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Gene: tena, bsu11650. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Tetramer (from PQS)

Resolution:

2.40Å

R-factor:

0.212

R-free:

0.257

Authors:

S.S.Rajan,L.Shuvalova,X.Yang,W.F.Anderson,Midwest Center For Structural Genomics (Mcsg)

Key ref:

S.S.Rajan et al. Crystal structure of thi-4 protein from bacillus subtilis. To be published, .

Date:

14-Jun-04

Release date:

10-Aug-04

PROCHECK

	Headers

	References

Protein chains

P25052 (TENA_BACSU) - Aminopyrimidine aminohydrolase from Bacillus subtilis (strain 168)

Seq: Struc:					236 a.a. 225 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.5.99.2 - aminopyrimidine aminohydrolase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	4-amino-5-aminomethyl-2-methylpyrimidine + H2O = 4-amino-5- hydroxymethyl-2-methylpyrimidine + NH4⁺
2.	thiamine + H2O = 5-(2-hydroxyethyl)-4-methylthiazole + 4-amino-5- hydroxymethyl-2-methylpyrimidine + H⁺

4-amino-5-aminomethyl-2-methylpyrimidine	+	H2O	=	4-amino-5- hydroxymethyl-2-methylpyrimidine	+	NH4(+)

thiamine

H2O

5-(2-hydroxyethyl)-4-methylthiazole
Bound ligand (Het Group name = HMH)
corresponds exactly

4-amino-5- hydroxymethyl-2-methylpyrimidine

H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site