PDBsum entry 1rc0

Transferase

PDB id

1rc0

Loading ...

Contents

			Protein chains

					200 a.a. *

			Ligands

					KT5 ×2

			Waters ×155

* Residue conservation analysis

PDB id:

1rc0

Links

Name:

Transferase

Title:

Human gar tfase complex structure with polyglutamated 10- (trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid

Structure:

Phosphoribosylglycinamide formyltransferase. Chain: a, b. Fragment: (residues 808-1010). Synonym: gart. Gar transformylase. 5'-phosphoribosylglycinamide transformylase. Engineered: yes. Other_details: part of trifunctional purine biosynthetic protein adenosine-3

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: purn. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Dimer (from PQS)

Resolution:

2.05Å

R-factor:

0.223

R-free:

0.243

Authors:

Y.Zhang,J.Desharnais,D.L.Boger,I.A.Wilson

Key ref:

Y.Zhang et al. Human gar tfase complex structure. To be published, .

Date:

03-Nov-03

Release date:

14-Jun-05

PROCHECK

	Headers

	References

Protein chains

P22102 (PUR2_HUMAN) - Trifunctional purine biosynthetic protein adenosine-3 from Homo sapiens

Seq: Struc:

Seq: Struc:					1010 a.a. 200 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class 1:

E.C.2.1.2.2 - phosphoribosylglycinamide formyltransferase 1.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Purine Biosynthesis (early stages)

Reaction:

N₁-(5-phospho-beta-D-ribosyl)glycinamide + (6R)-10- formyltetrahydrofolate = N₂-formyl-N₁-(5-phospho-beta-D- ribosyl)glycinamide + (6S)-5,6,7,8-tetrahydrofolate + H⁺

10-formyltetrahydrofolate	+	N(1)-(5-phospho-D-ribosyl)glycinamide	=	tetrahydrofolate	+	N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide

Enzyme class 2:

E.C.6.3.3.1 - phosphoribosylformylglycinamidine cyclo-ligase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Reaction:

2-formamido-N₁-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino- 1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H⁺

2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine	+	ATP	=	5-amino- 1-(5-phospho-beta-D-ribosyl)imidazole	+	ADP	+	phosphate	+	H(+)

Cofactor:

Magnesium

Enzyme class 3:

E.C.6.3.4.13 - phosphoribosylamine--glycine ligase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Pathway:

Reaction:

5-phospho-beta-D-ribosylamine + glycine + ATP = N₁-(5-phospho-beta-D- ribosyl)glycinamide + ADP + phosphate + H⁺

5-phospho-beta-D-ribosylamine	+	glycine	+	ATP	=	N(1)-(5-phospho-beta-D- ribosyl)glycinamide	+	ADP	+	phosphate	+	H(+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site