PDBsum entry 1qrw

Hydrolase

PDB id: 1qrw

Contents

Protein chain

198 a.a. *

Ligands

SO4 ×4

GOL ×2

Waters ×225

* Residue conservation analysis

PDB id:

1qrw

Name:

Hydrolase

Title:

Crystal structure of an alpha-lytic protease mutant with accelerated folding kinetics, r102h/g134s, ph 8

Structure:

Alhpa-lytic protease. Chain: a. Fragment: mature protease domain, residues 1-198. Engineered: yes. Mutation: yes

Source:

Lysobacter enzymogenes. Organism_taxid: 69. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.20Å R-factor: 0.177 R-free: 0.181

Authors:

A.I.Derman,T.Mau,D.A.Agard

Key ref:

A.I.Derman et al. A genetic screen that targets specifically the folding transition state of alpha-Lytic protease. To be published, .

Date:

16-Jun-99 Release date: 18-Jun-99

Protein chain

P00778  (PRLA_LYSEN) -  Alpha-lytic protease from Lysobacter enzymogenes

Seq: 397 a.a.

Struc: 198 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.21.12 - alpha-lytic endopeptidase.
• Reaction: Hydrolysis of proteins, especially bonds adjacents to L-alanine and L-valine residues in bacterial cell walls, elastin and other proteins.