PDBsum entry 1pi3

Lyase

PDB id: 1pi3

Contents

Protein chain

523 a.a. *

Ligands

TZD

Metals

_MG

_CA ×3

Waters ×504

* Residue conservation analysis

PDB id:

1pi3

Name:

Lyase

Title:

E28q mutant benzoylformate decarboxylase from pseudomonas putida

Structure:

Benzoylformate decarboxylase. Chain: a. Synonym: bfd, bfdc. Engineered: yes. Mutation: yes

Source:

Pseudomonas putida. Organism_taxid: 303. Gene: mdlc. Expressed in: escherichia coli. Expression_system_taxid: 562.

Biol. unit:

Tetramer (from PDB file)

Resolution:

1.20Å R-factor: 0.121 R-free: 0.136

Authors:

A.K.Bera,M.S.Hasson

Key ref:

A.K.Bera and m.s.hasson High resolution structure of benzoylformate decarboxylate e28q mutant. To be published, .

Date:

29-May-03 Release date: 23-Nov-04

Protein chain

P20906  (MDLC_PSEPU) -  Benzoylformate decarboxylase from Pseudomonas putida

Seq: 528 a.a.

Struc: 523 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.4.1.1.7 - benzoylformate decarboxylase.
• Reaction: phenylglyoxylate + H⁺ = benzaldehyde + CO2
• Cofactor: Thiamine diphosphate

Thiamine diphosphate (Bound ligand (Het Group name = TZD) matches with 96.30% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site