PDBsum entry 1oi4

Pfpi/thij family

PDB id: 1oi4

Contents

Protein chains

192 a.a. *

174 a.a. *

Waters ×404

* Residue conservation analysis

PDB id:

1oi4

Name:

Pfpi/thij family

Title:

Crystal structure of yhbo from escherichia coli

Structure:

Hypothetical protein yhbo. Chain: a, b. Engineered: yes. Other_details: 21 more residues in nter (gateway system)

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Expressed in: escherichia coli. Expression_system_taxid: 469008. Other_details: n-terminal poly-histidine tag (21 residues)

Biol. unit:

Dimer (from PDB file)

Resolution:

2.03Å R-factor: 0.187 R-free: 0.237

Authors:

J.B.Claude,C.Abergel,J.M.Claverie

Key ref:

J.B.Claude et al. Crystal structure of yhbo from escherichia coli. To be published, .

Date:

06-Jun-03 Release date: 03-Jul-03

Protein chain

P45470  (YHBO_ECOLI) -  Protein/nucleic acid deglycase 2 from Escherichia coli (strain K12)

Seq: 172 a.a.

Struc: 192 a.a.*

Protein chain

P45470  (YHBO_ECOLI) -  Protein/nucleic acid deglycase 2 from Escherichia coli (strain K12)

Seq: 172 a.a.

Struc: 174 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.3.1.2.- - ?????
• Enzyme class 3: Chains A, B: E.C.3.5.1.- - ?????
• Enzyme class 4: Chains A, B: E.C.3.5.1.124 - protein deglycase.
• Reaction:
  1. N(omega)-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] + H2O = lactate + L-arginyl-[protein] + H⁺
  2. N₆-(1-hydroxy-2-oxopropyl)-L-lysyl-[protein] + H2O = lactate + L-lysyl-[protein] + H⁺
  3. S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] + H2O = lactate + L-cysteinyl-[protein] + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site