PDBsum entry 1of0

Oxidoreductase

PDB id: 1of0

Contents

Protein chain

503 a.a. *

Ligands

C1O

OXY

GOL ×2

EBS

Metals

CU1 ×3

Waters ×238

* Residue conservation analysis

PDB id:

1of0

Name:

Oxidoreductase

Title:

Crystal structure of bacillus subtilis cota after 1h soaking with abts

Structure:

Spore coat protein a. Chain: a. Synonym: cota. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

2.45Å R-factor: 0.197 R-free: 0.252

Authors:

F.J.Enguita,D.Marcal,R.Grenha,L.O.Martins,A.O.Henriques,M.A.Carrondo

Key ref:

F.J.Enguita et al. Structural characterization of a bacterial laccase reaction cycle. To be published, .

Date:

03-Apr-03 Release date: 21-May-04

Protein chain

P07788  (COTA_BACSU) -  Laccase from Bacillus subtilis (strain 168)

Seq: 513 a.a.

Struc: 503 a.a.

Enzyme reactions

• Enzyme class 1: E.C.1.10.3.2 - laccase.
• Reaction: 4 hydroquinone + O2 = 4 benzosemiquinone + 2 H2O

4 × hydroquinone + O2 (Bound ligand (Het Group name = OXY) corresponds exactly) = 4 × benzosemiquinone + 2 × H2O

• Cofactor: Cu cation
• Enzyme class 2: E.C.1.3.3.5 - bilirubin oxidase.

Pathway:

• Reaction: 2 (4Z,15Z)-bilirubin IXalpha + O2 = 2 biliverdin IXalpha + 2 H2O

2 × (4Z,15Z)-bilirubin IXalpha + O2 (Bound ligand (Het Group name = OXY) corresponds exactly) = 2 × biliverdin IXalpha + 2 × H2O

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site