PDBsum entry 1mh2

Hydrolase

PDB id: 1mh2

Contents

Protein chains

119 a.a. *

119 a.a. *

Ligands

ACY ×2

Metals

_ZN

Waters ×184

* Residue conservation analysis

PDB id:

1mh2

Name:

Hydrolase

Title:

Crystal structure of a zinc containing dimer of phospholipase a2 from the venom of indian cobra (naja naja sagittifera)

Structure:

Phospholipase a2. Chain: a. Phospholipase a2. Chain: b. Ec: 3.1.1.4

Source:

Naja sagittifera. Organism_taxid: 195058. Secretion: venom. Secretion: venom

Resolution:

2.70Å R-factor: 0.196 R-free: 0.216

Authors:

T.Jabeen,A.K.Varma,M.Paramasivam,N.Singh,R.K.Singh,S.Sharma, A.Srinivasan,T.P.Singh

Key ref:

T.Jabeen et al. Crystal structure of a zinc containing dimer of phospholipase a2 from the venom of indian cobra (naja naja saggittifera). To be published, .

Date:

19-Aug-02 Release date: 20-May-03

Protein chain

P60043  (PA2B1_NAJSG) -  Basic phospholipase A2 1 (Fragment) from Naja sagittifera

Seq: 126 a.a.

Struc: 119 a.a.*

Protein chain

P60044  (PA2A2_NAJSG) -  Acidic phospholipase A2 2 (Fragment) from Naja sagittifera

Seq: 126 a.a.

Struc: 119 a.a.*

* PDB and UniProt seqs differ at 14 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.1.1.4 - phospholipase A2.
• Reaction: a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3- phosphocholine + a fatty acid + H⁺

1,2-diacyl-sn-glycero-3-phosphocholine + H2O = 1-acyl-sn-glycero-3- phosphocholine + fatty acid (Bound ligand (Het Group name = ACY) matches with 60.00% similarity) + H(+)

• Cofactor: Ca(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site