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PDBsum entry 1mal
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Outer membrane protein PDB id
1mal

 

 

 

 

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Contents
Protein chains
421 a.a. *
* Residue conservation analysis
PDB id:
1mal
Name: Outer membrane protein
Title: Structural basis for sugar translocation through maltoporin channels at 3.1 angstroms resolution
Structure: Maltoporin. Chain: a, b, c. Synonym: lamb
Source: Escherichia coli. Organism_taxid: 562. Organelle: outer membrane
Biol. unit: Trimer (from PQS)
Resolution:
3.10Å     R-factor:   0.217     R-free:   0.240
Authors: T.Schirmer
Key ref: T.Schirmer et al. (1995). Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution. Science, 267, 512-514. PubMed id: 7824948 DOI: 10.1126/science.7824948
Date:
24-Nov-94     Release date:   31-Dec-95    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P02943  (LAMB_ECOLI) -  Maltoporin from Escherichia coli (strain K12)
Seq:
Struc: 		446 a.a.
421 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1126/science.7824948 Science 267:512-514 (1995)
PubMed id: 7824948  
 
 
Structural basis for sugar translocation through maltoporin channels at 3.1 A resolution.
T.Schirmer, T.A.Keller, Y.F.Wang, J.P.Rosenbusch.
 
  ABSTRACT  
 
Trimeric maltoporin (LamB protein) facilitates the diffusion of maltodextrins across the outer membrane of Gram-negative bacteria. The crystal structure of maltoporin from Escherichia coli, determined to a resolution of 3.1 angstroms, reveals an 18-stranded, antiparallel beta-barrel that forms the framework of the channel. Three inwardly folded loops contribute to a constriction about halfway through the channel. Six contingent aromatic residues line the channel and form a path from the vestibule to the periplasmic outlet. Soaking of a crystal with maltotriose revealed binding of the sugar to this hydrophobic track across the constriction, which suggests that maltose and linear oligosaccharides may be translocated across the membrane by guided diffusion along this path.
 

Literature references that cite this PDB file's key reference

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Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity.
  J Bacteriol, 181, 1920-1923.  
10089413 C.Vonrhein, and G.E.Schulz (1999).
Locating proper non-crystallographic symmetry in low-resolution electron-density maps with the program GETAX.
  Acta Crystallogr D Biol Crystallogr, 55, 225-229.  
10048033 F.J.Bauer, T.Rudel, M.Stein, and T.F.Meyer (1999).
Mutagenesis of the Neisseria gonorrhoeae porin reduces invasion in epithelial cells and enhances phagocyte responsiveness.
  Mol Microbiol, 31, 903-913.  
12194172 G.Bains, and T.Lithgow (1999).
The Tom channel in the mitochondrial outer membrane: alive and kicking.
  Bioessays, 21, 1-4.  
  9864305 H.Nikaido (1999).
Microdermatology: cell surface in the interaction of microbes with the external world.
  J Bacteriol, 181, 4-8.  
10611277 K.E.McAuley, P.K.Fyfe, J.P.Ridge, N.W.Isaacs, R.J.Cogdell, and M.R.Jones (1999).
Structural details of an interaction between cardiolipin and an integral membrane protein.
  Proc Natl Acad Sci U S A, 96, 14706-14711.
PDB code: 1qov
10026305 K.Saxena, V.Drosou, E.Maier, R.Benz, and B.Ludwig (1999).
Ion selectivity reversal and induction of voltage-gating by site-directed mutations in the Paracoccus denitrificans porin.
  Biochemistry, 38, 2206-2212.  
10209114 M.S.Sansom (1999).
Membrane proteins: A tale of barrels and corks.
  Curr Biol, 9, R254-R257.  
10449368 S.K.Buchanan (1999).
Beta-barrel proteins from bacterial outer membranes: structure, function and refolding.
  Curr Opin Struct Biol, 9, 455-461.  
10383757 S.M.Newton, J.D.Igo, D.C.Scott, and P.E.Klebba (1999).
Effect of loop deletions on the binding and transport of ferric enterobactin by FepA.
  Mol Microbiol, 32, 1153-1165.  
10089392 T.Tomizaki, E.Yamashita, H.Yamaguchi, H.Aoyama, T.Tsukihara, K.Shinzawa-Itoh, R.Nakashima, R.Yaono, and S.Yoshikawa (1999).
Structure analysis of bovine heart cytochrome c oxidase at 2.8 A resolution.
  Acta Crystallogr D Biol Crystallogr, 55, 31-45.  
10383762 U.W.Könninger, S.Hobbie, R.Benz, and V.Braun (1999).
The haemolysin-secreting ShlB protein of the outer membrane of Serratia marcescens: determination of surface-exposed residues and formation of ion-permeable pores by ShlB mutants in artificial lipid bilayer membranes.
  Mol Microbiol, 32, 1212-1225.  
11498352 V.Braun (1999).
Active transport of siderophore-mimicking antibacterials across the outer membrane.
  Drug Resist Updat, 2, 363-369.  
  9684898 A.D.Ferguson, J.Breed, K.Diederichs, W.Welte, and J.W.Coulton (1998).
An internal affinity-tag for purification and crystallization of the siderophore receptor FhuA, integral outer membrane protein from Escherichia coli K-12.
  Protein Sci, 7, 1636-1638.  
9726928 A.Mathes, and H.Engelhardt (1998).
Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes.
  Biophys J, 75, 1255-1262.  
9631547 A.Prilipov, P.S.Phale, P.Van Gelder, J.P.Rosenbusch, and R.Koebnik (1998).
Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins from E. coli.
  FEMS Microbiol Lett, 163, 65-72.  
  9642192 A.Prilipov, P.S.Phale, R.Koebnik, C.Widmer, and J.P.Rosenbusch (1998).
Identification and characterization of two quiescent porin genes, nmpC and ompN, in Escherichia coli BE.
  J Bacteriol, 180, 3388-3392.  
9437417 B.K.Jap, and P.J.Walian (1998).
Gliding through sugar channels: how sweet it is!
  Nat Struct Biol, 5, 6-8.  
  9683466 C.Baldermann, A.Lupas, J.Lubieniecki, and H.Engelhardt (1998).
The regulated outer membrane protein Omp21 from Comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties.
  J Bacteriol, 180, 3741-3749.  
  9748429 C.J.Lazdunski, E.Bouveret, A.Rigal, L.Journet, R.Lloubès, and H.Bénédetti (1998).
Colicin import into Escherichia coli cells.
  J Bacteriol, 180, 4993-5002.  
  9620961 C.L.Cosma, M.D.Crotwell, S.Y.Burrows, and T.J.Silhavy (1998).
Folding-based suppression of extracytoplasmic toxicity conferred by processing-defective LamB.
  J Bacteriol, 180, 3120-3130.  
  9655352 C.S.Klug, and J.B.Feix (1998).
Guanidine hydrochloride unfolding of a transmembrane beta-strand in FepA using site-directed spin labeling.
  Protein Sci, 7, 1469-1476.  
9636045 C.S.Klug, S.S.Eaton, G.R.Eaton, and J.B.Feix (1998).
Ligand-induced conformational change in the ferric enterobactin receptor FepA as studied by site-directed spin labeling and time-domain ESR.
  Biochemistry, 37, 9016-9023.  
9437428 D.Forst, W.Welte, T.Wacker, and K.Diederichs (1998).
Structure of the sucrose-specific porin ScrY from Salmonella typhimurium and its complex with sucrose.
  Nat Struct Biol, 5, 37-46.
PDB codes: 1a0s 1a0t
9635733 D.P.Tieleman, and H.J.Berendsen (1998).
A molecular dynamics study of the pores formed by Escherichia coli OmpF porin in a fully hydrated palmitoyloleoylphosphatidylcholine bilayer.
  Biophys J, 74, 2786-2801.  
9782060 E.Gouaux (1998).
Single potassium ion seeks open channel for transmembrane travels: tales from the KcsA structure.
  Structure, 6, 1221-1226.  
10089518 H.Kim (1998).
Crystallization of OmpC osmoporin from Escherichia coli.
  Acta Crystallogr D Biol Crystallogr, 54, 1399-1400.  
9778731 I.R.Henderson, F.Navarro-Garcia, and J.P.Nataro (1998).
The great escape: structure and function of the autotransporter proteins.
  Trends Microbiol, 6, 370-378.  
9920390 I.T.Arkin, and A.T.Brunger (1998).
Statistical analysis of predicted transmembrane alpha-helices.
  Biochim Biophys Acta, 1429, 113-128.  
9558483 K.Kirk, and K.Strange (1998).
Functional properties and physiological roles of organic solute channels.
  Annu Rev Physiol, 60, 719-739.  
  9761484 K.Seshadri, R.Garemyr, E.Wallin, G.von Heijne, and A.Elofsson (1998).
Architecture of beta-barrel membrane proteins: analysis of trimeric porins.
  Protein Sci, 7, 2026-2032.  
9437421 P.C.Preusch, J.C.Norvell, J.C.Cassatt, and M.Cassman (1998).
Progress away from 'no crystals, no grant'.
  Nat Struct Biol, 5, 12-14.  
10066479 P.E.Klebba, and S.M.Newton (1998).
Mechanisms of solute transport through outer membrane porins: burning down the house.
  Curr Opin Microbiol, 1, 238-247.  
  9852016 P.Thulasiraman, S.M.Newton, J.Xu, K.N.Raymond, C.Mai, A.Hall, M.A.Montague, and P.E.Klebba (1998).
Selectivity of ferric enterobactin binding and cooperativity of transport in gram-negative bacteria.
  J Bacteriol, 180, 6689-6696.  
9751640 R.M.Garavito (1998).
Membrane protein structures: the known world expands.
  Curr Opin Biotechnol, 9, 344-349.  
  9529892 W.Boos, and H.Shuman (1998).
Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.
  Microbiol Mol Biol Rev, 62, 204-229.  
9853688 Y.V.Matsuka, D.A.Dilts, S.Hoiseth, and R.Arumugham (1998).
Characterization of a subunit structure and stability of the recombinant porin from Neisseria gonorrhoeae.
  J Protein Chem, 17, 719-728.  
9241431 C.Chothia, T.Hubbard, S.Brenner, H.Barns, and A.Murzin (1997).
Protein folds in the all-beta and all-alpha classes.
  Annu Rev Biophys Biomol Struct, 26, 597-627.  
9335564 C.S.Klug, W.Su, and J.B.Feix (1997).
Mapping of the residues involved in a proposed beta-strand located in the ferric enterobactin receptor FepA using site-directed spin-labeling.
  Biochemistry, 36, 13027-13033.  
  9294435 C.Ulmke, J.W.Lengeler, and K.Schmid (1997).
Identification of a new porin, RafY, encoded by raffinose plasmid pRSD2 of Escherichia coli.
  J Bacteriol, 179, 5783-5788.  
9294168 D.R.Liu, T.J.Magliery, M.Pastrnak, and P.G.Schultz (1997).
Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo.
  Proc Natl Acad Sci U S A, 94, 10092-10097.  
  9397533 F.Clementi (1997).
Alginate production by Azotobacter vinelandii.
  Crit Rev Biotechnol, 17, 327-361.  
  9401026 G.Merino, and H.A.Shuman (1997).
Unliganded maltose-binding protein triggers lactose transport in an Escherichia coli mutant with an alteration in the maltose transport system.
  J Bacteriol, 179, 7687-7694.  
9345760 H.Mobasheri, T.A.Ficht, H.Marquis, E.J.Lea, and J.H.Lakey (1997).
Brucella Omp2a and Omp2b porins: single channel measurements and topology prediction.
  FEMS Microbiol Lett, 155, 23-30.  
9266177 J.B.Heymann, D.J.Müller, K.Mitsuoka, and A.Engel (1997).
Electron and atomic force microscopy of membrane proteins.
  Curr Opin Struct Biol, 7, 543-549.  
  9144780 J.E.Meyer, and G.E.Schulz (1997).
Energy profile of maltooligosaccharide permeation through maltoporin as derived from the structure and from a statistical analysis of saccharide-protein interactions.
  Protein Sci, 6, 1084-1091.  
  9006035 J.Maurer, J.Jose, and T.F.Meyer (1997).
Autodisplay: one-component system for efficient surface display and release of soluble recombinant proteins from Escherichia coli.
  J Bacteriol, 179, 794-804.  
9345617 J.Rini, and K.Drickamer (1997).
Carbohydrates and glycoconjugates.
  Curr Opin Struct Biol, 7, 615-616.  
9288896 K.P.Locher, and J.P.Rosenbusch (1997).
Oligomeric states and siderophore binding of the ligand-gated FhuA protein that forms channels across Escherichia coli outer membranes.
  Eur J Biochem, 247, 770-775.  
9129811 M.Watanabe, J.Rosenbusch, T.Schirmer, and M.Karplus (1997).
Computer simulations of the OmpF porin from the outer membrane of Escherichia coli.
  Biophys J, 72, 2094-2102.  
9138559 O.S.Smart, J.Breed, G.R.Smith, and M.S.Sansom (1997).
A novel method for structure-based prediction of ion channel conductance properties.
  Biophys J, 72, 1109-1126.  
9266175 R.M.Garavito, and S.H.White (1997).
Membrane proteins. Structure, assembly, and function: a panoply of progress.
  Curr Opin Struct Biol, 7, 533-536.  
9114029 S.M.Newton, J.S.Allen, Z.Cao, Z.Qi, X.Jiang, C.Sprencel, J.D.Igo, S.B.Foster, M.A.Payne, and P.E.Klebba (1997).
Double mutagenesis of a positive charge cluster in the ligand-binding site of the ferric enterobactin receptor, FepA.
  Proc Natl Acad Sci U S A, 94, 4560-4565.  
9157886 X.Jiang, M.A.Payne, Z.Cao, S.B.Foster, J.B.Feix, S.M.Newton, and P.E.Klebba (1997).
Ligand-specific opening of a gated-porin channel in the outer membrane of living bacteria.
  Science, 276, 1261-1264.  
  8655519 B.H.Rehm, and R.E.Hancock (1996).
Membrane topology of the outer membrane protein OprH from Pseudomonas aeruginosa: PCR-mediated site-directed insertion and deletion mutagenesis.
  J Bacteriol, 178, 3346-3349.  
9132415 C.A.Mannella, A.F.Neuwald, and C.E.Lawrence (1996).
Detection of likely transmembrane beta strand regions in sequences of mitochondrial pore proteins using the Gibbs sampler.
  J Bioenerg Biomembr, 28, 163-169.  
  8655484 H.Huang, and R.E.Hancock (1996).
The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginosa.
  J Bacteriol, 178, 3085-3090.  
8643551 H.W.Sun, J.Bernhagen, R.Bucala, and E.Lolis (1996).
Crystal structure at 2.6-A resolution of human macrophage migration inhibitory factor.
  Proc Natl Acad Sci U S A, 93, 5191-5196.
PDB code: 1mif
  8955391 I.Martin-Verstraete, V.Michel, and A.Charbit (1996).
The levanase operon of Bacillus subtilis expressed in Escherichia coli can substitute for the mannose permease in mannose uptake and bacteriophage lambda infection.
  J Bacteriol, 178, 7112-7119.  
8785323 J.Breed, R.Sankararamakrishnan, I.D.Kerr, and M.S.Sansom (1996).
Molecular dynamics simulations of water within models of ion channels.
  Biophys J, 70, 1643-1661.  
  8617231 M.Bonhivers, A.Ghazi, P.Boulanger, and L.Letellier (1996).
FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5.
  EMBO J, 15, 1850-1856.  
8744306 M.Cheung, and M.H.Akabas (1996).
Identification of cystic fibrosis transmembrane conductance regulator channel-lining residues in and flanking the M6 membrane-spanning segment.
  Biophys J, 70, 2688-2695.  
  8844839 M.Przybylski, M.O.Glocker, U.Nestel, V.Schnaible, M.Blüggel, K.Diederichs, J.Weckesser, M.Schad, A.Schmid, W.Welte, and R.Benz (1996).
X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: implications for ion selectivity and single-channel conductance.
  Protein Sci, 5, 1477-1489.  
  8606171 N.A.Linderoth, P.Model, and M.Russel (1996).
Essential role of a sodium dodecyl sulfate-resistant protein IV multimer in assembly-export of filamentous phage.
  J Bacteriol, 178, 1962-1970.  
8916906 P.Boulanger, M.le Maire, M.Bonhivers, S.Dubois, M.Desmadril, and L.Letellier (1996).
Purification and structural and functional characterization of FhuA, a transporter of the Escherichia coli outer membrane.
  Biochemistry, 35, 14216-14224.  
8755503 P.R.Chipman, M.Agbandje-McKenna, S.Kajigaya, K.E.Brown, N.S.Young, T.S.Baker, and M.G.Rossmann (1996).
Cryo-electron microscopy studies of empty capsids of human parvovirus B19 complexed with its cellular receptor.
  Proc Natl Acad Sci U S A, 93, 7502-7506.  
8730870 R.Chen, and U.Henning (1996).
A periplasmic protein (Skp) of Escherichia coli selectively binds a class of outer membrane proteins.
  Mol Microbiol, 19, 1287-1294.  
8805519 R.Dutzler, Y.F.Wang, P.Rizkallah, J.P.Rosenbusch, and T.Schirmer (1996).
Crystal structures of various maltooligosaccharides bound to maltoporin reveal a specific sugar translocation pathway.
  Structure, 4, 127-134.
PDB codes: 1mpm 1mpn 1mpo
  8636026 R.Ehrle, C.Pick, R.Ulrich, E.Hofmann, and M.Ehrmann (1996).
Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.
  J Bacteriol, 178, 2255-2262.  
  8786233 R.M.Garavito, D.Picot, and P.J.Loll (1996).
Strategies for crystallizing membrane proteins.
  J Bioenerg Biomembr, 28, 13-27.  
8692877 S.C.Li, N.K.Goto, K.A.Williams, and C.M.Deber (1996).
Alpha-helical, but not beta-sheet, propensity of proline is determined by peptide environment.
  Proc Natl Acad Sci U S A, 93, 6676-6681.  
  8655540 S.M.Newton, P.E.Klebba, V.Michel, M.Hofnung, and A.Charbit (1996).
Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.
  J Bacteriol, 178, 3447-3456.  
  9147661 W.C.Chan, T.Schirmer, and T.Ferenci (1996).
Combinatorial mutagenesis analysis of residues in the channel constriction loop L3 and neighbouring beta-strands in the LamB glycoporin of Escherichia coli.
  Mol Membr Biol, 13, 41-48.  
8592709 A.Hirsch, T.Wacker, J.Weckesser, K.Diederichs, and W.Welte (1995).
Purification, characterization, crystallization, and preliminary X-ray results from Paracoccus denitrificans porin.
  Proteins, 23, 282-284.  
  7545149 A.Sukhan, and R.E.Hancock (1995).
Insertion mutagenesis of the Pseudomonas aeruginosa phosphate-specific porin OprP.
  J Bacteriol, 177, 4914-4920.  
8590000 E.Pebay-Peyroula, R.M.Garavito, J.P.Rosenbusch, M.Zulauf, and P.A.Timmins (1995).
Detergent structure in tetragonal crystals of OmpF porin.
  Structure, 3, 1051-1059.  
7579649 J.L.Popot, and M.Saraste (1995).
Engineering membrane proteins.
  Curr Opin Biotechnol, 6, 394-402.  
  7592472 J.T.Lathrop, B.Y.Wei, G.A.Touchie, and R.J.Kadner (1995).
Sequences of the Escherichia coli BtuB protein essential for its insertion and function in the outer membrane.
  J Bacteriol, 177, 6810-6819.  
8574701 M.G.Rossmann (1995).
Ab initio phase determination and phase extension using non-crystallographic symmetry.
  Curr Opin Struct Biol, 5, 650-655.  
8528766 M.Montal (1995).
Molecular mimicry in channel-protein structure.
  Curr Opin Struct Biol, 5, 501-506.  
8534803 M.S.Sansom, and I.D.Kerr (1995).
Transbilayer pores formed by beta-barrels: molecular modeling of pore structures and properties.
  Biophys J, 69, 1334-1343.  
7480624 R.Grisshammer, and C.G.Tate (1995).
Overexpression of integral membrane proteins for structural studies.
  Q Rev Biophys, 28, 315-422.  
7538310 R.MacKinnon (1995).
Pore loops: an emerging theme in ion channel structure.
  Neuron, 14, 889-892.  
8618841 S.Choe, C.F.Stevens, and J.M.Sullivan (1995).
Three distinct structural environments of a transmembrane domain in the inwardly rectifying potassium channel ROMK1 defined by perturbation.
  Proc Natl Acad Sci U S A, 92, 12046-12049.  
8589999 S.W.Cowan, R.M.Garavito, J.N.Jansonius, J.A.Jenkins, R.Karlsson, N.König, E.F.Pai, R.A.Pauptit, P.J.Rizkallah, and J.P.Rosenbusch (1995).
The structure of OmpF porin in a tetragonal crystal form.
  Structure, 3, 1041-1050.
PDB codes: 1opf 1opu 1opv
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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