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PDBsum entry 1ffp
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Immune system/signaling protein
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PDB id
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1ffp
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Immune system/signaling protein
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Title:
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Crystal structure of murine class i h-2db complexed with peptide gp33 (c9m/k1s)
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Structure:
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H-2 class i histocompatibility antigen, d-b, alpha chain. Chain: a, d. Fragment: extracellular portion. Synonym: h-2d(b). Engineered: yes. Beta-2 microglobulin beta chain. Chain: b, e. Fragment: beta-2 microglobulin. Engineered: yes.
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Synthetic: yes. Other_details: the peptide was synthesized
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Biol. unit:
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Trimer (from
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Resolution:
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2.60Å
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R-factor:
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0.250
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R-free:
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0.303
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Authors:
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B.Wang,A.Sharma,R.Maile,M.Saad,E.J.Collins,J.A.Frelinger
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Key ref:
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B.Wang
et al.
(2002).
Peptidic termini play a significant role in TCR recognition.
J Immunol,
169,
3137-3145.
PubMed id:
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Date:
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25-Jul-00
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Release date:
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11-Dec-02
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PROCHECK
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Headers
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References
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J Immunol
169:3137-3145
(2002)
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PubMed id:
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Peptidic termini play a significant role in TCR recognition.
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B.Wang,
A.Sharma,
R.Maile,
M.Saad,
E.J.Collins,
J.A.Frelinger.
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ABSTRACT
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TCR recognition of class I MHC is dependent on the composition of the antigenic
peptide and the MHC. Single amino acid substitutions in either the MHC or the
peptide may dramatically alter recognition. While the major interactions between
TCR and the peptide/MHC complex appear to be focused on the
complementarity-determining region (CDR)3, it is also clear from the cocrystal
structure of class I MHC and TCR that the amino and carboxyl ends of the peptide
may play a role through interactions with the CDR1. In this work we show that
gp33 variants substituted at the peptidic termini at the putative CDR1 contact
regions show improved recognition in B6 mice. The rank order of recognition is
different using the P14 transgenic T cells, suggesting that one reason for
improved recognition is a change in the TCR repertoire that recognizes the
peptide. However, the affinity of the TCR by some of the peptide/MHC complex
with increased recognition is improved, as shown by increased tetramer binding
to P14 T cells. These substitutions at the termini of the peptide-binding cleft
cause localized conformational changes as seen by changes in mAb binding and
crystallographic structures. The different peptide structures also show
different conformations in the center of the peptide, but these are shown to be
energetically similar and thus most likely have no significance with respect to
TCR recognition. Therefore, small conformational changes, localized to the CDR1
contact regions, may play a significant role in TCR recognition.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.Bidot,
F.Gruy,
C.S.Haudin,
F.El Hentati,
B.Guy,
and
C.Lambert
(2008).
Mathematical modeling of T-cell activation kinetic.
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J Comput Biol,
15,
105-128.
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J.Racape,
F.Connan,
J.Hoebeke,
J.Choppin,
and
J.G.Guillet
(2006).
Influence of dominant HIV-1 epitopes on HLA-A3/peptide complex formation.
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Proc Natl Acad Sci U S A,
103,
18208-18213.
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J.L.Chen,
G.Stewart-Jones,
G.Bossi,
N.M.Lissin,
L.Wooldridge,
E.M.Choi,
G.Held,
P.R.Dunbar,
R.M.Esnouf,
M.Sami,
J.M.Boulter,
P.Rizkallah,
C.Renner,
A.Sewell,
P.A.van der Merwe,
B.K.Jakobsen,
G.Griffiths,
E.Y.Jones,
and
V.Cerundolo
(2005).
Structural and kinetic basis for heightened immunogenicity of T cell vaccines.
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J Exp Med,
201,
1243-1255.
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PDB codes:
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M.J.Miley,
I.Messaoudi,
B.M.Metzner,
Y.Wu,
J.Nikolich-Zugich,
and
D.H.Fremont
(2004).
Structural basis for the restoration of TCR recognition of an MHC allelic variant by peptide secondary anchor substitution.
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J Exp Med,
200,
1445-1454.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
