PDBsum entry 1dp3

DNA binding protein

PDB id

1dp3

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Contents

			Protein chain

					55 a.a. *

* Residue conservation analysis

PDB id:

1dp3

Links
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Name:

DNA binding protein

Title:

Solution structure of the DNA binding domain of the tram protein

Structure:

Tram protein. Chain: a. Fragment: tramm26. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 562. Expressed in: escherichia coli. Expression_system_taxid: 562.

NMR struc:

20 models

Authors:

T.Stockner,C.Plugariu,G.Koraimann,G.Hoegenauer,W.Bermel,S.Prytulla, H.Sterk

Key ref:

T.Stockner et al. (2001). Solution structure of the DNA-binding domain of TraM. Biochemistry, 40, 3370-3377. PubMed id: 11258958 DOI: 10.1021/bi002031c

Date:

23-Dec-99

Release date:

04-Apr-01

PROCHECK

	Headers

	References

Protein chain

P07294 (TRAM2_ECOLX) - Relaxosome protein TraM from Escherichia coli

Seq: Struc:					127 a.a. 55 a.a.

Key:

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.?

[IntEnz] [ExPASy] [KEGG] [BRENDA]

DOI no: 10.1021/bi002031c	Biochemistry 40:3370-3377 (2001)
PubMed id: 11258958

Solution structure of the DNA-binding domain of TraM.
T.Stockner, C.Plugariu, G.Koraimann, G.Högenauer, W.Bermel, S.Prytulla, H.Sterk.

ABSTRACT

The solution structure of the DNA-binding domain of the TraM protein, an essential component of the DNA transfer machinery of the conjugative resistance plasmid R1, is presented. The structure has been determined using homonuclear 2-dimensional NMR spectroscopy as well as 15N labeled heteronuclear 2- and 3-dimensional NMR spectroscopy. It turns out that the solution structure of the DNA binding domain of the TraM protein is globular and dominantly helical. The very first amino acids of the N-terminus are unstructured.

Literature references that cite this PDB file's key reference

PubMed id

Reference

17079132

F.X.Gomis-Rüth, and M.Coll (2006).
Cut and move: protein machinery for DNA processing in bacterial conjugation.

Curr Opin Struct Biol, 16, 744-752.

16710295

J.Lu, R.A.Edwards, J.J.Wong, J.Manchak, P.G.Scott, L.S.Frost, and J.N.Glover (2006).
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TraM.

EMBO J, 25, 2930-2939.

PDB codes:

2g7o 2g9e

15466052

A.Beranek, M.Zettl, K.Lorenzoni, A.Schauer, M.Manhart, and G.Koraimann (2004).
Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM.

J Bacteriol, 186, 6999-7006.

12529360

D.L.Miller, and J.F.Schildbach (2003).
Evidence for a monomeric intermediate in the reversible unfolding of F factor TraM.

J Biol Chem, 278, 10400-10407.

11748238

F.X.Gomis-Rüth, G.Moncalían, F.de la Cruz, and M.Coll (2002).
Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein. Detailed structural features and mapping of the active site cleft.

J Biol Chem, 277, 7556-7566.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.