› 8uvk

X-ray diffraction
2.21Å resolution

CosR DNA bound form II

Released:
DOI: 10.2210/pdb8uvk/pdb
PDB entry 8uvk coloured by chain, front view.
PDB entry 8uvk coloured by chain, side view.
PDB entry 8uvk coloured by chain, top view.
Source organism: Campylobacter jejuni
Primary publication:
Structural basis of DNA recognition of the Campylobacter jejuni CosR regulator.
Zhang Z, Yan Y, Pang J, Dai L, Zhang Q, Yu EW
mBio 15 e0343023 (2024)
PMID: 38323832

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-239283 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeostatic response regulator transcription factor HsrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 224 amino acids
Theoretical weight: 25.56 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A3H9R6A1 (Residues: 2-223; Coverage: 100%)
Gene names: CO830_02550, hsrA
Sequence domains:
DNA (5'-D(*TP*TP*GP*GP*TP*TP*AP*AP*TP*AP*AP*TP*AP*TP*CP*TP*TP*AP*AP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Campylobacter jejuni
Expression system: Not provided
DNA (5'-D(*AP*AP*TP*TP*AP*AP*GP*AP*TP*AP*TP*TP*AP*TP*TP*AP*AP*CP*CP*AP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Campylobacter jejuni
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 66.62Å b: 80.101Å c: 68.202Å
α: 90° β: 105.6° γ: 90°
R-values:
R R work R free
0.254 0.253 0.279
Expression systems:
  • Escherichia coli
  • Not provided

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