Function and Biology Details
Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero tridecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-243391 (preferred)
Entry contents:
10 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (13 distinct):
DNA-directed RNA polymerase II subunit RPB1
Chain: A
Molecule details ›
DNA-directed RNA polymerase II subunit RPB2
Chain: B
Molecule details ›
DNA-directed RNA polymerase II subunit RPB3
Chain: C
Molecule details ›
Chain: C
Length: 318 amino acids
Theoretical weight: 35.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains:
Length: 318 amino acids
Theoretical weight: 35.33 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P16370 (Residues: 1-318; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC1
Chain: E
Molecule details ›
DNA-directed RNA polymerases I, II, and III subunit RPABC2
Chain: F
Molecule details ›
Chain: F
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains: RNA polymerase Rpb6
Length: 155 amino acids
Theoretical weight: 17.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P20435 (Residues: 1-155; Coverage: 100%)
Sequence domains: RNA polymerase Rpb6
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Chain: H
Molecule details ›
Chain: H
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains: RNA polymerase Rpb8
Length: 146 amino acids
Theoretical weight: 16.53 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P20436 (Residues: 1-146; Coverage: 100%)
Sequence domains: RNA polymerase Rpb8
DNA-directed RNA polymerase II subunit RPB9
Chain: I
Molecule details ›
Chain: I
Length: 122 amino acids
Theoretical weight: 14.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains:
Length: 122 amino acids
Theoretical weight: 14.31 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P27999 (Residues: 1-122; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC5
Chain: J
Molecule details ›
Chain: J
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains: RNA polymerases N / 8 kDa subunit
Length: 70 amino acids
Theoretical weight: 8.29 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P22139 (Residues: 1-70; Coverage: 100%)
Sequence domains: RNA polymerases N / 8 kDa subunit
DNA-directed RNA polymerase II subunit RPB11
Chain: K
Molecule details ›
Chain: K
Length: 120 amino acids
Theoretical weight: 13.63 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains:
Length: 120 amino acids
Theoretical weight: 13.63 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P38902 (Residues: 1-120; Coverage: 100%)
Sequence domains:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Chain: L
Molecule details ›
Chain: L
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Saccharomyces cerevisiae S288C
UniProt:
- Canonical: P40422 (Residues: 1-70; Coverage: 100%)
Sequence domains: DNA directed RNA polymerase, 7 kDa subunit
tsDNA with Fapy-dG lesion
Chain: T
Molecule details ›
Chain: T
Length: 29 nucleotides
Theoretical weight: 8.76 KDa
Length: 29 nucleotides
Theoretical weight: 8.76 KDa
ntsDNA
Chain: N
Molecule details ›
Chain: N
Length: 18 nucleotides
Theoretical weight: 5.66 KDa
Length: 18 nucleotides
Theoretical weight: 5.66 KDa
RNA
Chain: R
Molecule details ›
Chain: R
Length: 10 nucleotides
Theoretical weight: 3.26 KDa
Length: 10 nucleotides
Theoretical weight: 3.26 KDa
Ligands and Environments
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 5.0.1
Spacegroup:
C2
Unit cell:
a: 156.681Å
b: 221.393Å
c: 190.539Å
α: 90°
β: 97.67°
γ: 90°
R-values:
R
R work
R free
0.252
0.251
0.29
Expression system: Saccharomyces cerevisiae S288C