› 8uhb

Electron Microscopy
3.35Å resolution

Cryo-EM Structure of the Ro5256390-bound hTA1-Gs heterotrimer signaling complex

Released:
DOI: 10.2210/pdb8uhb/pdb
PDB entry 8uhb coloured by chain, front view.
PDB entry 8uhb coloured by chain, side view.
PDB entry 8uhb coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis of human trace amine-associated receptor 1 activation
Zilberg G, Parpounas AK, Warren AL, Yang S, Wacker D
Nat Commun 15 108- (2024)
Related structures: EMD-42268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-238690 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Beta-2 adrenergic receptor; Soluble cytochrome b562; Trace amine-associated receptor 1 Chain: A
Molecule details ›
Chain: A
Length: 524 amino acids
Theoretical weight: 60.02 KDa
Source organisms: Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P0ABE7 (Residues: 23-123; Coverage: 95%)
  • Canonical: P07550 (Residues: 1-30; Coverage: 7%)
  • Canonical: Q96RJ0 (Residues: 1-339; Coverage: 100%)
Gene names: ADRB2, ADRB2R, B2AR, TA1, TAAR1, TAR1, TRAR1, cybC
Sequence domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 358 amino acids
Theoretical weight: 39.42 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 7-340; Coverage: 98%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 394 amino acids
Theoretical weight: 45.8 KDa
Source organism: Rattus norvegicus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63095 (Residues: 1-394; Coverage: 100%)
Gene names: Gnas, Gnas1
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 134 amino acids
Theoretical weight: 14.71 KDa
Source organism: Lama glama
Expression system: Spodoptera frugiperda

Ligands and Environments

1 bound ligand:
Ligand WV8 1 x WV8
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.35Å
Relevant EMDB volumes: EMD-42268
Expression system: Spodoptera frugiperda

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