› 8sv8

Electron Microscopy
3.38Å resolution

Cryo-EM structure of a double loaded human UBA7-UBE2L6-ISG15 thioester mimetic complex from a composite map

Released:
DOI: 10.2210/pdb8sv8/pdb
PDB entry 8sv8 coloured by chain, front view.
PDB entry 8sv8 coloured by chain, side view.
PDB entry 8sv8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer.
Afsar M, Liu G, Jia L, Ruben EA, Nayak D, Sayyad Z, Bury PDS, Cano KE, Nayak A, Zhao XR, Shukla A, Sung P, Wasmuth EV, Gack MU, Olsen SK
Nat Commun 14 4786 (2023)
PMID: 37553340
Related structures: EMD-40782

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-231664 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin-like modifier-activating enzyme 7 Chain: A
Molecule details ›
Chain: A
Length: 1012 amino acids
Theoretical weight: 111.82 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P41226 (Residues: 1-1012; Coverage: 100%)
Gene names: UBA7, UBE1L, UBE2
Sequence domains:
Ubiquitin-like protein ISG15 Chains: B, D
Molecule details ›
Chains: B, D
Length: 157 amino acids
Theoretical weight: 17.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05161 (Residues: 1-157; Coverage: 95%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family
Ubiquitin/ISG15-conjugating enzyme E2 L6 Chain: C
Molecule details ›
Chain: C
Length: 152 amino acids
Theoretical weight: 17.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14933 (Residues: 2-153; Coverage: 99%)
Gene names: UBCH8, UBE2L6
Sequence domains: Ubiquitin-conjugating enzyme

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.38Å
Relevant EMDB volumes: EMD-40782
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Insights into the ISG15 transfer cascade by the UBE1L activating enzyme.
Wallace et al. (2023)