› 8sh1

X-ray diffraction
2.6Å resolution

Structure of human POT1 DNA binding domain bound to a 5'-phosphorylated junction of a telomeric double-stranded DNA duplex with a 3'-overhang

Released:
DOI: 10.2210/pdb8sh1/pdb
PDB entry 8sh1 coloured by chain, front view.
PDB entry 8sh1 coloured by chain, side view.
PDB entry 8sh1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human POT1 protects the telomeric ds-ss DNA junction by capping the 5' end of the chromosome.
Tesmer VM, Brenner KA, Nandakumar J
Science 381 771-778 (2023)
PMID: 37590346

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227602 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Protection of telomeres protein 1 Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.81 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9NUX5 (Residues: 1-299; Coverage: 47%)
Gene name: POT1
Sequence domains:
DNA (5'-D(*CP*GP*CP*GP*CP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.88 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(P*CP*TP*AP*AP*CP*GP*CP*GP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I222
Unit cell:
a: 58.47Å b: 170.62Å c: 173.96Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.254
Expression systems:
  • Trichoplusia ni
  • Not provided

Quick links