› 8qtt

X-ray diffraction
2.35Å resolution

Crystal structure of a C-terminally truncated version of Arabidopsis thaliana 14-3-3 omega in complex with a phosphopeptide from the inhibitor protein BKI1.

Released:
DOI: 10.2210/pdb8qtt/pdb
PDB entry 8qtt coloured by chain, front view.
PDB entry 8qtt coloured by chain, side view.
PDB entry 8qtt coloured by chain, top view.
Source organism: Arabidopsis thaliana
Entry authors: Hothorn M, Obergfell E

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235904 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3-like protein GF14 omega Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 241 amino acids
Theoretical weight: 27.14 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q01525 (Residues: 1-237; Coverage: 92%)
Gene names: At1g78300, F3F9.16, GF14, GRF2
Sequence domains: 14-3-3 protein
BRI1 kinase inhibitor 1 Chains: K, L, M, N, O, P, Q, R, S, T
Molecule details ›
Chains: K, L, M, N, O, P, Q, R, S, T
Length: 6 amino acids
Theoretical weight: 743 Da
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21

Ligands and Environments

2 bound ligands:
Ligand FMT 10 x FMT
Ligand EDO 1 x EDO
1 modified residue:
Ligand SEP 10 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P1
Unit cell:
a: 71.216Å b: 71.082Å c: 150.986Å
α: 100.47° β: 95.53° γ: 88.74°
R-values:
R R work R free
0.236 0.234 0.276
Expression system: Escherichia coli BL21

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