› 8poh

Electron Microscopy
3.3Å resolution

Influenza A/H7N9 polymerase symmetric dimer bound to the promoter (PA K289A/C489R)

Released:
DOI: 10.2210/pdb8poh/pdb
PDB entry 8poh coloured by chain, front view.
PDB entry 8poh coloured by chain, side view.
PDB entry 8poh coloured by chain, top view.
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Primary publication:
The host RNA polymerase II C-terminal domain is the anchor for replication of the influenza virus genome.
Krischuns T, Arragain B, Isel C, Paisant S, Budt M, Wolff T, Cusack S, Naffakh N
Nat Commun 15 1064 (2024)
PMID: 38316757
Related structures: EMD-17792

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-240174 (preferred)
Entry contents:
3 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
Polymerase acidic protein Chains: A, E
Molecule details ›
Chains: A, E
Length: 729 amino acids
Theoretical weight: 84.39 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TI86 (Residues: 1-716; Coverage: 100%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chains: B, F
Molecule details ›
Chains: B, F
Length: 757 amino acids
Theoretical weight: 86.5 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: M9TLW3 (Residues: 1-757; Coverage: 100%)
Gene name: PB1
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PB1
Polymerase basic protein 2 Chains: C, G
Molecule details ›
Chains: C, G
Length: 788 amino acids
Theoretical weight: 88.94 KDa
Source organism: Influenza A virus (A/Zhejiang/DTID-ZJU01/2013(H7N9))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: X5F427 (Residues: 1-759; Coverage: 100%)
Gene name: PB2
Sequence domains:
51-mer vRNA loop (v51_mut_S) Chains: R, S, U, V
Molecule details ›
Chains: R, S, U, V
Length: 51 nucleotides
Theoretical weight: 16.09 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17792
Expression system: Trichoplusia ni

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