› 8pi4

X-ray diffraction
1.25Å resolution

Crystal structure of human insulin desB30 precursor with an Alanine-Methionine-Lysine C-peptide in dimer (T2) conformation

Released:
DOI: 10.2210/pdb8pi4/pdb
PDB entry 8pi4 coloured by chain, front view.
PDB entry 8pi4 coloured by chain, side view.
PDB entry 8pi4 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular engineering of insulin for recombinant expression in yeast.
Kjeldsen T, Andersen AS, Hubálek F, Johansson E, Kreiner FF, Schluckebier G, Kurtzhals P
Trends Biotechnol (2023)
PMID: 37880066

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235014 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin B chain Chain: A
Molecule details ›
Chain: A
Length: 53 amino acids
Theoretical weight: 6.03 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P01308 (Residues: 25-53, 90-110; Coverage: 58%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I213
Unit cell:
a: 77.4Å b: 77.4Å c: 77.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.176
Expression system: Saccharomyces cerevisiae

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