8p94

Electron Microscopy
3.3Å resolution

Cryo-EM structure of cortactin stabilized Arp2/3-complex nucleated actin branches

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 30-mer (preferred)
PDBe Complex ID:
PDB-CPX-237934 (preferred)
Entry contents:
12 distinct polypeptide molecules
Macromolecules (12 distinct):
Actin-related protein 3 Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.43 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P61158 (Residues: 1-418; Coverage: 100%)
Gene names: ACTR3, ARP3
Sequence domains: Actin
Actin-related protein 2 Chain: B
Molecule details ›
Chain: B
Length: 394 amino acids
Theoretical weight: 44.82 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P61160 (Residues: 1-394; Coverage: 100%)
Gene names: ACTR2, ARP2
Sequence domains: Actin
Actin-related protein 2/3 complex subunit 1B Chain: C
Molecule details ›
Chain: C
Length: 372 amino acids
Theoretical weight: 41 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: O15143 (Residues: 1-372; Coverage: 100%)
Gene names: ARC41, ARPC1B
Sequence domains: WD domain, G-beta repeat
Actin-related protein 2/3 complex subunit 2 Chain: D
Molecule details ›
Chain: D
Length: 300 amino acids
Theoretical weight: 34.39 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: O15144 (Residues: 1-300; Coverage: 100%)
Gene names: ARC34, ARPC2, PRO2446
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Actin-related protein 2/3 complex subunit 3 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 20.57 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: O15145 (Residues: 1-178; Coverage: 100%)
Gene names: ARC21, ARPC3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Actin-related protein 2/3 complex subunit 4 Chain: F
Molecule details ›
Chain: F
Length: 168 amino acids
Theoretical weight: 19.7 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: P59998 (Residues: 1-168; Coverage: 100%)
Gene names: ARC20, ARPC4
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Actin-related protein 2/3 complex subunit 5-like protein Chain: G
Molecule details ›
Chain: G
Length: 153 amino acids
Theoretical weight: 16.96 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q9BPX5 (Residues: 1-153; Coverage: 100%)
Gene name: ARPC5L
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Actin, cytoplasmic 1 Chains: H, J, K, N, O, P, Q, R, S, W
Molecule details ›
Chains: H, J, K, N, O, P, Q, R, S, W
Length: 375 amino acids
Theoretical weight: 41.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q6QAQ1 (Residues: 1-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Src substrate cortactin Chain: I
Molecule details ›
Chain: I
Length: 546 amino acids
Theoretical weight: 61.34 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60598 (Residues: 1-546; Coverage: 100%)
Gene names: Cttn, Ems1
Sequence domains:
F-actin-capping protein subunit alpha-1 Chain: U
Molecule details ›
Chain: U
Length: 286 amino acids
Theoretical weight: 32.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47753 (Residues: 1-286; Coverage: 100%)
Gene names: Cappa1, Capza1
Sequence domains: F-actin capping protein alpha subunit
F-actin-capping protein subunit beta Chain: V
Molecule details ›
Chain: V
Length: 272 amino acids
Theoretical weight: 30.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P47757 (Residues: 1-270; Coverage: 98%)
Gene names: Cappb1, Capzb
Sequence domains: F-actin capping protein, beta subunit
Phalloidin Chains: a, b, c, d, h, i, j, k, l, m
Molecule details ›
Chains: a, b, c, d, h, i, j, k, l, m
Length: 7 amino acids
Theoretical weight: 809 Da
Source organism: Amanita phalloides

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-17558
Expression systems:
  • unidentified baculovirus
  • Escherichia coli