› 8jjc

X-ray diffraction
2.76Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-242262 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 431 amino acids
Theoretical weight: 48.3 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-431; Coverage: 97%)
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 189 amino acids
Theoretical weight: 22.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 1-189; Coverage: 100%)
Gene name: Stmn4
Sequence domains: Stathmin family
TTL Chain: F
Molecule details ›
Chain: F
Length: 380 amino acids
Theoretical weight: 43.83 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli

Ligands and Environments

8 bound ligands:
Ligand GTP 3 x GTP
Ligand MG 5 x MG
Ligand CA 6 x CA
Ligand MES 1 x MES
Ligand UPO 1 x UPO
Ligand GDP 1 x GDP
Ligand CL 1 x CL
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 104.493Å b: 157.005Å c: 180.398Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.235 0.264
Expression system: Escherichia coli

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