› 8jfr

X-ray diffraction
3.1Å resolution

N-terminal domain of AcrIIA15 in complex with palindromic DNA substrate

Released:
DOI: 10.2210/pdb8jfr/pdb
PDB entry 8jfr coloured by chain, front view.
PDB entry 8jfr coloured by chain, side view.
PDB entry 8jfr coloured by chain, top view.
Source organism: Staphylococcus delphini
Entry authors: Deng X, Wang Y

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-240445 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
AcrIIA15 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 63 amino acids
Theoretical weight: 7.1 KDa
Source organism: Staphylococcus delphini
Expression system: Escherichia coli BL21(DE3)
DNA (5'-D(*AP*TP*TP*AP*TP*GP*AP*CP*AP*AP*AP*TP*GP*TP*CP*AP*TP*AP*G)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Staphylococcus delphini
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*AP*TP*GP*AP*CP*AP*TP*TP*TP*GP*TP*CP*AP*TP*AP*A)-3') Chains: F, H
Molecule details ›
Chains: F, H
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: Staphylococcus delphini
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P43
Unit cell:
a: 58.712Å b: 58.712Å c: 240.593Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.246 0.259
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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