› 8j7y

Electron Microscopy
3.4Å resolution

Cryo-EM structure of hZnT7DeltaHis-loop-Fab complex in zinc-bound state, determined in outward-facing conformation

Released:
DOI: 10.2210/pdb8j7y/pdb
PDB entry 8j7y coloured by chain, front view.
PDB entry 8j7y coloured by chain, side view.
PDB entry 8j7y coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structures of human zinc transporter ZnT7 reveal the mechanism of Zn2+ uptake into the Golgi apparatus.
Bui HB, Watanabe S, Nomura N, Liu K, Uemura T, Inoue M, Tsutsumi A, Fujita H, Kinoshita K, Kato Y, Iwata S, Kikkawa M, Inaba K
Nat Commun 14 4770 (2023)
PMID: 37553324
Related structures: EMD-36053

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-229582 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Zinc transporter 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 390 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q8NEW0 (Residues: 1-376; Coverage: 100%)
Gene names: SLC30A7, ZNT7, ZNTL2
Sequence domains: Cation efflux family
Heavy chain of YN7114-08 Fab Chains: E, F
Molecule details ›
Chains: E, F
Length: 234 amino acids
Theoretical weight: 24.97 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Light chain of YN7114-08 Fab Chains: C, D
Molecule details ›
Chains: C, D
Length: 218 amino acids
Theoretical weight: 24.14 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-36053
Expression systems:
  • Homo sapiens
  • Mus musculus

Quick links

Citations

1 citations in other articles

Energy coupling and stoichiometry of Zn2+/H+ antiport by the prokaryotic cation diffusion facilitator YiiP.
Hussein et al. (2023)