› 8j3o

X-ray diffraction
2.65Å resolution

Formate dehydrogenase wild-type enzyme from Candida dubliniensis complexed with NADH

Released:
DOI: 10.2210/pdb8j3o/pdb
PDB entry 8j3o coloured by chain, front view.
PDB entry 8j3o coloured by chain, side view.
PDB entry 8j3o coloured by chain, top view.
Source organism: Candida dubliniensis CD36
Primary publication:
Engineering a Formate Dehydrogenase for NADPH Regeneration.
Ma W, Geng Q, Chen C, Zheng YC, Yu HL, Xu JH
Chembiochem e202300390 (2023)
PMID: 37455264

Function and Biology Details

Reaction catalysed: 
Formate + NAD(+) = CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-228946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Formate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 386 amino acids
Theoretical weight: 43 KDa
Source organism: Candida dubliniensis CD36
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B9WHT3 (Residues: 1-379; Coverage: 100%)
Gene names: CD36_53500, Cd36_53500
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand NAI 4 x NAI
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P1
Unit cell:
a: 51.402Å b: 60.156Å c: 120.482Å
α: 90° β: 90° γ: 111.02°
R-values:
R R work R free
0.229 0.226 0.278
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Changing the Electron Acceptor Specificity of Rhodobacter capsulatus Formate Dehydrogenase from NAD+ to NADP.
Kumar et al. (2023)