› 8itv

X-ray diffraction
2.3Å resolution

KL2.1 in complex with CRM1-Ran-RanBP1

Released:
DOI: 10.2210/pdb8itv/pdb
PDB entry 8itv coloured by chain, front view.
PDB entry 8itv coloured by chain, side view.
PDB entry 8itv coloured by chain, top view.
Source organisms:
Entry authors: Sun Q, Jian L

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-226618 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
GTP-binding nuclear protein Ran Chain: A
Molecule details ›
Chain: A
Length: 216 amino acids
Theoretical weight: 24.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
YRB1 isoform 1 Chain: B
Molecule details ›
Chain: B
Length: 140 amino acids
Theoretical weight: 16.32 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6A5PZB5 (Residues: 62-201; Coverage: 70%)
Gene names: GI527_G0000925, YRB1
Sequence domains: RanBP1 domain
CRM1 isoform 1 Chain: C
Molecule details ›
Chain: C
Length: 1003 amino acids
Theoretical weight: 115.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6A5PZI8 (Residues: 1-1058; Coverage: 92%)
Gene names: CRM1, GI527_G0002657
Sequence domains:

Ligands and Environments

8 bound ligands:
Ligand MG 1 x MG
Ligand NO3 14 x NO3
Ligand DMS 9 x DMS
Ligand GOL 5 x GOL
Ligand GTP 1 x GTP
Ligand CL 1 x CL
Ligand ACT 1 x ACT
Ligand Q73 1 x Q73
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P43212
Unit cell:
a: 106.014Å b: 106.014Å c: 305.893Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.224
Expression system: Escherichia coli

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