› 8hmm

X-ray diffraction
2.7Å resolution

Crystal structure of AoRhaA

Released:
DOI: 10.2210/pdb8hmm/pdb
PDB entry 8hmm coloured by chain, front view.
PDB entry 8hmm coloured by chain, side view.
PDB entry 8hmm coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Aspergillus oryzae α-l-rhamnosidase: Crystal structure and insight into the substrate specificity.
Makabe K, Ishida N, Kanezaki N, Shiono Y, Koseki T
Proteins (2023)
PMID: 37818702

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-236343 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha-L-rhamnosidase Chain: A
Molecule details ›
Chain: A
Length: 642 amino acids
Theoretical weight: 69.38 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2UJB1 (Residues: 22-663; Coverage: 100%)
Gene name: AO090003001291
Sequence domains: Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain

Ligands and Environments

1 bound ligand:
Ligand NAG 5 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 61.67Å b: 95.868Å c: 111.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.241 0.266
Expression system: Escherichia coli

Quick links