› 8g5n

Electron Microscopy
2.73Å resolution

Cryo-EM structure of the Guide loop Engagement Complex (VI) of Human Mitochondrial DNA Polymerase Gamma

Released:
DOI: 10.2210/pdb8g5n/pdb
PDB entry 8g5n coloured by chain, front view.
PDB entry 8g5n coloured by chain, side view.
PDB entry 8g5n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA proofreading.
Buchel G, Nayak AR, Herbine K, Sarfallah A, Sokolova VO, Zamudio-Ochoa A, Temiakov D
Nat Commun 14 8501 (2023)
PMID: 38151585
Related structures: EMD-29750

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-238580 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
1 distinct RNA molecule
Macromolecules (4 distinct):
DNA polymerase subunit gamma-1 Chain: A
Molecule details ›
Chain: A
Length: 1239 amino acids
Theoretical weight: 139.63 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P54098 (Residues: 1-1239; Coverage: 100%)
Gene names: MDP1, POLG, POLG1, POLGA
Sequence domains: DNA mitochondrial polymerase exonuclease domain
DNA polymerase subunit gamma-2 Chains: B, C
Molecule details ›
Chains: B, C
Length: 485 amino acids
Theoretical weight: 54.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHN1 (Residues: 1-485; Coverage: 100%)
Gene names: MTPOLB, POLG2
Sequence domains: Anticodon binding domain
Template DNA Chain: T
Molecule details ›
Chain: T
Length: 30 nucleotides
Theoretical weight: 9.18 KDa
Mismatched RNA primer Chain: R
Molecule details ›
Chain: R
Length: 24 nucleotides
Theoretical weight: 7.86 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.73Å
Relevant EMDB volumes: EMD-29750
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

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