› 8f3z

X-ray diffraction
2.8Å resolution

Crystal structure of Penicillin Binding Protein 5 (PBP5) S422A variant apo form from Enterococcus faecium

Released:
DOI: 10.2210/pdb8f3z/pdb
PDB entry 8f3z coloured by chain, front view.
PDB entry 8f3z coloured by chain, side view.
PDB entry 8f3z coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
The Molecular Basis for Resistance of E. faecium PBP5 to beta-lactam Antibiotics
Hunashal Y, Kumar GS, Choy MS, Da Silva Santiago A, D'Andrea ED, Schoenle MV, Arthur M, Rice LB, Page R, Peti W
Nat Commun (2023)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235540 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein Chain: A
Molecule details ›
Chain: A
Length: 645 amino acids
Theoretical weight: 70.1 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1X4JFA2 (Residues: 37-678; Coverage: 95%)
Gene name: EFM1CSP_06200
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 24 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P6322
Unit cell:
a: 190.692Å b: 190.692Å c: 156.108Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Escherichia coli BL21(DE3)

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