› 8ej0

X-ray diffraction
2.59Å resolution

Crystal structure of Fe-S cluster-dependent dehydratase from Paralcaligenes ureilyticus in complex with Mg

Released:
DOI: 10.2210/pdb8ej0/pdb
PDB entry 8ej0 coloured by chain, front view.
PDB entry 8ej0 coloured by chain, side view.
PDB entry 8ej0 coloured by chain, top view.
Source organism: Paralcaligenes ureilyticus
Entry authors: Bayaraa T, Lonhienne T, Guddat LW

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227524 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroxyacid dehydratase Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 575 amino acids
Theoretical weight: 62.65 KDa
Source organism: Paralcaligenes ureilyticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A4R3LQ44 (Residues: 1-575; Coverage: 100%)
Gene name: EDC26_11947
Sequence domains: Dehydratase family

Ligands and Environments

4 bound ligands:
Ligand FES 4 x FES
Ligand MG 4 x MG
Ligand BCT 6 x BCT
Ligand CO2 1 x CO2
1 modified residue:
Ligand KCX 4 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 69.908Å b: 178.071Å c: 92.858Å
α: 90° β: 101.72° γ: 90°
R-values:
R R work R free
0.195 0.194 0.239
Expression system: Escherichia coli BL21(DE3)

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