› 8ei2

X-ray diffraction
2.8Å resolution

Crystal structure of the N-terminal domain of CUL5 in complex with H314, a Helicon Polypeptide

Released:
DOI: 10.2210/pdb8ei2/pdb
PDB entry 8ei2 coloured by chain, front view.
PDB entry 8ei2 coloured by chain, side view.
PDB entry 8ei2 coloured by chain, top view.
Source organisms:
Primary publication:
Recognition and reprogramming of E3 ubiquitin ligase surfaces by α-helical peptides.
Tokareva OS, Li K, Travaline TL, Thomson TM, Swiecicki JM, Moussa M, Ramirez JD, Litchman S, Verdine GL, McGee JH
Nat Commun 14 6992 (2023)
PMID: 37914719

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-232992 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cullin-5 Chain: A
Molecule details ›
Chain: A
Length: 387 amino acids
Theoretical weight: 44.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93034 (Residues: 1-386; Coverage: 50%)
Gene names: CUL5, VACM1
Sequence domains: Cullin family
H314 Chain: C
Molecule details ›
Chain: C
Length: 18 amino acids
Theoretical weight: 1.87 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand WHL 1 x WHL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P6
Unit cell:
a: 173.03Å b: 173.03Å c: 57.48Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.34 0.339 0.358
Expression systems:
  • Escherichia coli
  • Not provided

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