› 8eb2

X-ray diffraction
2.9Å resolution

Structure of HLA-A*02:01 in complex with NY-ESO-1 peptide and PA2.1 Fab

Released:
DOI: 10.2210/pdb8eb2/pdb
PDB entry 8eb2 coloured by chain, front view.
PDB entry 8eb2 coloured by chain, side view.
PDB entry 8eb2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
HLA-A∗02-gated safety switch for cancer therapy has exquisite specificity for its allelic target antigen.
Mock JY, Winters A, Riley TP, Bruno R, Naradikian MS, Sharma S, Jette CA, Elshimali R, Gahrs C, Toledo-Warshaviak D, West AP, Kamb A, Hamburger AE
Mol Ther Oncolytics 27 157-166 (2022)
PMID: 36381658

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-233074 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 275 amino acids
Theoretical weight: 31.85 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A140T9X5 (Residues: 25-299; Coverage: 79%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
NY-ESO-1 peptide Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 9 amino acids
Theoretical weight: 1.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
PA2.1 Fab Heavy Chain Chains: J, K, N
Molecule details ›
Chains: J, K, N
Length: 221 amino acids
Theoretical weight: 23.73 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
PA2.1 Fab Light Chain Chains: L, M, O
Molecule details ›
Chains: L, M, O
Length: 219 amino acids
Theoretical weight: 24.03 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 116.8Å b: 148.79Å c: 199.57Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.284
Expression systems:
  • Homo sapiens
  • Not provided

Quick links

Citations

1 mention without citation

HLA-A∗02-gated safety switch for cancer therapy has exquisite specificity for its allelic target antigen.
Mock et al. (2022)