› 8e6l

X-ray diffraction
3.12Å resolution

X-ray structure of the Deinococcus radiodurans Nramp/MntH divalent transition metal transporter D296A mutant in an inward-open, manganese-bound state

Released:
DOI: 10.2210/pdb8e6l/pdb
PDB entry 8e6l coloured by chain, front view.
PDB entry 8e6l coloured by chain, side view.
PDB entry 8e6l coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter.
Ray S, Berry SP, Wilson EA, Zhang CH, Shekhar M, Singharoy A, Gaudet R
Elife 12 (2023)
PMID: 37039477

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193268 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Divalent metal cation transporter MntH Chain: A
Molecule details ›
Chain: A
Length: 414 amino acids
Theoretical weight: 44.53 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RTP8 (Residues: 32-436; Coverage: 93%)
Gene names: DR_1709, mntH
Sequence domains: Natural resistance-associated macrophage protein-like

Ligands and Environments

4 bound ligands:
Ligand MN 1 x MN
Ligand CL 2 x CL
Ligand OLC 21 x OLC
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Unit cell:
a: 58.514Å b: 71.64Å c: 98.895Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.219 0.272
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Membrane Transporters Involved in Iron Trafficking: Physiological and Pathological Aspects.
Pasquadibisceglie et al. (2023)
1 more

1 mention without citation

High-resolution structures with bound Mn2+ and Cd2+ map the metal import pathway in an Nramp transporter.
Ray et al. (2023)