› 8e1m

Electron Microscopy
2.9Å resolution

Cryo-EM structure of the endogenous core TIM23 complex from S. cerevisiae

Released:
DOI: 10.2210/pdb8e1m/pdb
PDB entry 8e1m coloured by chain, front view.
PDB entry 8e1m coloured by chain, side view.
PDB entry 8e1m coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of mitochondrial protein import by the TIM23 complex.
Sim SI, Chen Y, Lynch DL, Gumbart JC, Park E
Nature (2023)
PMID: 37344598
Related structures: EMD-27825

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-235817 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Mitochondrial import inner membrane translocase subunit TIM17 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 16.6 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P39515 (Residues: 1-158; Coverage: 100%)
Gene names: J0648, MIM17, MPI2, SMS1, TIM17, YJL143W
Sequence domains: Tim17/Tim22/Tim23/Pmp24 family
Mitochondrial import inner membrane translocase subunit TIM23 Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 23.27 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32897 (Residues: 1-222; Coverage: 100%)
Gene names: MAS6, MIM23, MPI3, N3180, TIM23, YNR017W
Sequence domains: Tim17/Tim22/Tim23/Pmp24 family
Mitochondrial import inner membrane translocase subunit TIM44 Chain: C
Molecule details ›
Chain: C
Length: 431 amino acids
Theoretical weight: 48.93 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q01852 (Residues: 1-431; Coverage: 100%)
Gene names: ISP45, MIM44, MPI1, TIM44, YIL022W
Sequence domains: Tim44-like domain
Antibody Fab fragment light chain Chain: L
Molecule details ›
Chain: L
Length: 238 amino acids
Theoretical weight: 26.17 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Antibody Fab fragment heavy chain Chain: H
Molecule details ›
Chain: H
Length: 238 amino acids
Theoretical weight: 25.49 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

2 bound ligands:
Ligand CDL 1 x CDL
Ligand PTY 1 x PTY
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.9Å
Relevant EMDB volumes: EMD-27825
Expression system: Mus musculus

Quick links

Citations

1 review citation

Interactions of amyloidogenic proteins with mitochondrial protein import machinery in aging-related neurodegenerative diseases.
Reed et al. (2023)
3 other articles