› 8e0s

X-ray diffraction
1.65Å resolution

DAHP (3-deoxy-D-arabinoheptulosonate-7-phosphate) Synthase complexed with DAHP Oxime in unbound:(bound)2:unbound conformations

Released:
DOI: 10.2210/pdb8e0s/pdb
PDB entry 8e0s coloured by chain, front view.
PDB entry 8e0s coloured by chain, side view.
PDB entry 8e0s coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Role of Half-of-Sites Reactivity and Inter-Subunit Communications in DAHP Synthase Catalysis and Regulation.
Balachandran N, Grainger RA, Rob T, Liuni P, Wilson DJ, Junop MS, Berti PJ
Biochemistry (2022)
PMID: 36197914

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142030 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 351 amino acids
Theoretical weight: 38.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AB91 (Residues: 1-350; Coverage: 100%)
Gene names: JW0737, aroG, b0754
Sequence domains: DAHP synthetase I family

Ligands and Environments

3 bound ligands:
Ligand GLC 1 x GLC
Ligand 52L 2 x 52L
Ligand BGC 1 x BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: C2
Unit cell:
a: 209.881Å b: 53.127Å c: 150.415Å
α: 90° β: 115.435° γ: 90°
R-values:
R R work R free
0.185 0.185 0.199
Expression system: Escherichia coli BL21(DE3)

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