X-ray diffraction
2.1Å resolution

Dbr1 in complex with 5-mer cleavage product

Entry authors: Clark NE, Taylor AB

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Lariat debranching enzyme Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 356 amino acids
Theoretical weight: 41.21 KDa
Source organism: Entamoeba histolytica
Expression system: Escherichia coli
  • Canonical: C4M1P9 (Residues: 1-354; Coverage: 100%)
Gene names: DBR1, EHI_062730
Sequence domains: Calcineurin-like phosphoesterase
RNA (5'-R(P*(G46)P*UP*GP*UP*U)-3') Chains: F, G, N, O, P
Molecule details ›
Chains: F, G, N, O, P
Length: 5 nucleotides
Theoretical weight: 1.58 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 73.272Å b: 143.341Å c: 214.006Å
α: 90° β: 90° γ: 90°
R R work R free
0.179 0.178 0.209
Expression systems:
  • Escherichia coli
  • Not provided