8dh0

X-ray diffraction
2.9Å resolution

T7 RNA polymerase elongation complex with unnatural base dDs

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetradecamer (preferred)
PDBe Complex ID:
PDB-CPX-122505 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
T7 RNA polymerase Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 883 amino acids
Theoretical weight: 98.98 KDa
Source organism: Escherichia phage T7
UniProt:
  • Canonical: P00573 (Residues: 1-883; Coverage: 100%)
Gene name: 1
Sequence domains:
Template strand DNA Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 18 nucleotides
Theoretical weight: 5.62 KDa
Source organism: synthetic construct
Expression system: Not provided
Non-template strand DNA Chains: H, P
Molecule details ›
Chains: H, P
Length: 9 nucleotides
Theoretical weight: 2.67 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 12 nucleotides
Theoretical weight: 3.85 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-1
Spacegroup: P1
Unit cell:
a: 78.707Å b: 86.235Å c: 201.22Å
α: 89.91° β: 85.23° γ: 69.5°
R-values:
R R work R free
0.221 0.221 0.261
Expression system: Not provided