› 8db8

X-ray diffraction
2.21Å resolution

Adenosine/guanosine nucleoside hydrolase bound to ImH

Released:
DOI: 10.2210/pdb8db8/pdb
PDB 8db8 coloured by chain and viewed from the front.
PDB 8db8 coloured by chain and viewed from the side.
PDB 8db8 coloured by chain and viewed from the top.
Source organism: Trichomonas vaginalis
Primary publication:
Structure-Guided Insight into the Specificity and Mechanism of a Parasitic Nucleoside Hydrolase.
Muellers SN, Nyitray MM, Reynarowych N, Saljanin E, Benzie AL, Schoenfeld AR, Stockman BJ, Allen KN
Biochemistry (2022)
PMID: 35994320

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine/uridine-preferring nucleoside hydrolase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 304 amino acids
Theoretical weight: 33.48 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
UniProt:
  • Canonical: A2EYV3 (Residues: 1-304; Coverage: 100%)
Gene name: TVAG_213720
Sequence domains: Inosine-uridine preferring nucleoside hydrolase

Ligands and Environments

2 bound ligands:
Ligand IMH 2 x IMH
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P1
Unit cell:
a: 51.025Å b: 76.48Å c: 86.636Å
α: 75.479° β: 81.672° γ: 86.504°
R-values:
R R work R free
0.223 0.221 0.266
Expression system: Escherichia coli

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