8d1u

X-ray diffraction
1.3Å resolution

E. coli beta-ketoacyl-[acyl carrier protein] synthase III (FabH) with an acetylated cysteine and in complex with oxa(dethia)-Coenzyme A

Released:
Source organism: Escherichia coli
Entry authors: Benjamin AB, Stunkard LM, Ling J, Nice JN, Lohman JR

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-ketoacyl-[acyl-carrier-protein] synthase III Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 33.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A6R0 (Residues: 1-317; Coverage: 100%)
Gene names: JW1077, b1091, fabH
Sequence domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P41212
Unit cell:
a: 72.662Å b: 72.662Å c: 102.852Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.167
Expression system: Escherichia coli BL21(DE3)