› 8d0k

Electron Microscopy
4.27Å resolution

Human CST-DNA polymerase alpha/primase preinitiation complex bound to 4xTEL-foldback template - PRIM2C advanced PIC

Released:
DOI: 10.2210/pdb8d0k/pdb
PDB entry 8d0k coloured by chain, front view.
PDB entry 8d0k coloured by chain, side view.
PDB entry 8d0k coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of the human CST-Polα-primase complex bound to telomere templates.
He Q, Lin X, Chavez BL, Agrawal S, Lusk BL, Lim CJ
Nature (2022)
PMID: 35830881
Related structures: EMD-27107

Function and Biology Details

Reactions catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122493 (preferred)
Entry contents:
7 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (8 distinct):
CST complex subunit CTC1 Chain: A
Molecule details ›
Chain: A
Length: 1246 amino acids
Theoretical weight: 138.03 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q2NKJ3 (Residues: 2-1217; Coverage: 100%)
Gene names: C17orf68, CTC1
Sequence domains: CST, telomere maintenance, complex subunit CTC1
CST complex subunit STN1 Chain: B
Molecule details ›
Chain: B
Length: 374 amino acids
Theoretical weight: 43 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9H668 (Residues: 2-368; Coverage: 100%)
Gene names: OBFC1, STN1
Sequence domains:
CST complex subunit TEN1 Chain: C
Molecule details ›
Chain: C
Length: 152 amino acids
Theoretical weight: 17.29 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q86WV5 (Residues: 3-123; Coverage: 98%)
Gene names: C17orf106, TEN1
Sequence domains: Telomere-capping, CST complex subunit
DNA primase small subunit Chain: D
Molecule details ›
Chain: D
Length: 434 amino acids
Theoretical weight: 51.36 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49642 (Residues: 2-420; Coverage: 100%)
Gene name: PRIM1
Sequence domains: DNA primase small subunit
DNA primase large subunit Chain: E
Molecule details ›
Chain: E
Length: 523 amino acids
Theoretical weight: 60.27 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P49643 (Residues: 2-509; Coverage: 100%)
Gene names: PRIM2, PRIM2A
Sequence domains: Eukaryotic and archaeal DNA primase, large subunit
DNA polymerase alpha catalytic subunit Chain: F
Molecule details ›
Chain: F
Length: 1527 amino acids
Theoretical weight: 172.8 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P09884 (Residues: 2-1462; Coverage: 100%)
Gene names: POLA, POLA1
Sequence domains:
DNA polymerase alpha subunit B Chain: G
Molecule details ›
Chain: G
Length: 612 amino acids
Theoretical weight: 67.39 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q14181 (Residues: 2-598; Coverage: 100%)
Gene name: POLA2
Sequence domains:
DNA (5'-D(P*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*A)-3') Chain: H
Molecule details ›
Chain: H
Length: 60 nucleotides
Theoretical weight: 18.67 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.27Å
Relevant EMDB volumes: EMD-27107
Expression system: Trichoplusia ni

Quick links

Citations

6 review citations

CST-Polα/Primase: the second telomere maintenance machine.
Cai et al. (2023)
5 more

5 mentions without citation

Lagging Strand Initiation Processes in DNA Replication of Eukaryotes-Strings of Highly Coordinated Reactions Governed by Multiprotein Complexes.
Nasheuer et al. (2023)
4 more