› 8c8v

X-ray diffraction
2.2Å resolution

Priestia megaterium mupirocin-resistant isoleucyl-tRNA synthetase 2 with a fully-resolved C-terminal tRNA-binding domain complexed with an isoleucyl-adenylate analogue

Released:
DOI: 10.2210/pdb8c8v/pdb
PDB entry 8c8v coloured by chain, front view.
PDB entry 8c8v coloured by chain, side view.
PDB entry 8c8v coloured by chain, top view.
Source organism: Priestia megaterium NBRC 15308 = ATCC 14581
Primary publication:
Antibiotic hyper-resistance in a class I aminoacyl-tRNA synthetase with altered active site signature motif.
Brkic A, Leibundgut M, Jablonska J, Zanki V, Car Z, Petrovic Perokovic V, Marsavelski A, Ban N, Gruic-Sovulj I
Nat Commun 14 5498 (2023)
PMID: 37679387

Function and Biology Details

Reaction catalysed: 
ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-226560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isoleucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 1032 amino acids
Theoretical weight: 117.92 KDa
Source organism: Priestia megaterium NBRC 15308 = ATCC 14581
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0B6AVD3 (Residues: 1-1032; Coverage: 100%)
Gene names: BG04_5198, ileS
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ILA 1 x ILA
Ligand TAR 4 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P43212
Unit cell:
a: 108.15Å b: 108.15Å c: 240.18Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.229
Expression system: Escherichia coli BL21(DE3)

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