› 8c8c

X-ray diffraction
2.1Å resolution

Crystal Structure of Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PI5P4Ka) bound to an inhibitor

Released:
DOI: 10.2210/pdb8c8c/pdb
PDB entry 8c8c coloured by chain, front view.
PDB entry 8c8c coloured by chain, side view.
PDB entry 8c8c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Identification of ARUK2002821 as an isoform-selective PI5P4Kα inhibitor.
Willems HMG, Edwards S, Boffey HK, Chawner SJ, Green C, Romero T, Winpenny D, Skidmore J, Clarke JH, Andrews SP
RSC Med Chem 14 934-946 (2023)
PMID: 37252102

Function and Biology Details

Reaction catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155728 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 42.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48426 (Residues: 33-406; Coverage: 92%)
Gene names: PI5P4KA, PIP4K2A, PIP5K2, PIP5K2A
Sequence domains: Phosphatidylinositol-4-phosphate 5-Kinase

Ligands and Environments

1 bound ligand:
Ligand U1U 1 x U1U
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 70.661Å b: 84.935Å c: 128.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Lipid kinases PIP5Ks and PIP4Ks: potential drug targets for breast cancer.
Jin et al. (2023)
2 other articles

1 mention without citation

Identification of ARUK2002821 as an isoform-selective PI5P4Kα inhibitor.
Willems et al. (2023)